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| ID | Type | Description | Link |
|---|---|---|---|
| ChangGungMH | Registry Identifier | ChangGungMH |
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The team integrates experimental analyses of clinical and basic medicine and transgenic mice models. miR-29a acts a potent protective factor against excessive fibrosis in myofibroblasts of subacromial bursa tissue. Gain of miR-29a stabilizes tendon and synovial tissue homeostasis that alleviates tissue stiffness and maintains function.
Aims of first: The team will evaluate the associations among the mR-29a, miR-29b, miR-29c expression in subacromial bursa and subacromial fluid, incidence of shoulder stiffness, Constant scores, and VAS.
Aims of second: The team will isolate primary myofibroblast from subacromial bursa tissue as in vitro models and investigate the molecular events in the IL-1β modulation of miR-29a expression and the molecular mechanism underlying miR-29a inhibition of fibrotic matrix accumulation and apoptotic reactions in myofibroblast cultures.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| miR-29a precursor oligonucleotide | Experimental | Effects of IL-1β, miR-29a precursor oligonucleotide treatment on the expressions of miR-29a, miR-29b or miR-29c in cell cultures are quantified |
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| miR-29a antisense oligonucleotide | Experimental | Effects of IL-1β, miR-29a antisense oligonucleotide treatment on the expressions of miR-29a, miR-29b or miR-29c in cell cultures are quantified |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| miR 29a | Biological | Effects of IL-1β, miR-29a precursor or antisense oligonucleotide treatment on the expressions of miR-29a, miR-29b or miR-29c in cell cultures are quantified |
| Measure | Description | Time Frame |
|---|---|---|
| The microRNA expression, mRNA expression as a measure | Differences among incidence, microRNA expression, mRNA expression of shoulder stiffness are analyzed using non-parametric student's t-test. P value of < 0.05 is considered statistically significant. | 48hr |
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Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Jih-Yang Ko, MD | Contact | 886-7-731-7123 | 8003 | kojy@cgmh.org.tw |
| Name | Affiliation | Role |
|---|---|---|
| Yang-Jih Ko, MD | Chang Gung Memorial Hospital | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Chang Gung Memorial Hospital | Recruiting | Kaohsiung City | 833 | Taiwan |
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Double-blind