Bronchial Allergen Provocation Tests in Asthmatics in Guangzhou

Bronchial Provocation Tests With House Dust Mite in Allergic Asthma in Guangzhou

The purpose of this study is to establish the methodology of bronchial provocation tests with house dust mites in China, and to evaluate its safety and effects on upper and lower airways inflammation.

After screening,each participant undergo baseline lung function test,airway inflammation evaluation (blood and sputum eosinophils count,interleukin-13 (IL-13),FeNO)and airway responsiveness tests(methacholine bronchial provocation,nasal histamine provocation).

On the second day,the participant undergo bronchial house dust mites challenge tests.

On the third day,the participant undergo the lung function test,airway inflammation evaluation (blood and sputum eosinophils count,IL-13,FeNO)and airway responsiveness tests(methacholine bronchial provocation,nasal histamine provocation).

Inclusion Criteria:

• Clinical diagnosis of asthma;
• Sensitive to house dust mite;
• Stable for more than 4 weeks with FEV1≥70%pred

Exclusion Criteria:

• exacerbation within 3 months;
• upper airway infections within 6 weeks;
• concomitant of hypertension or heart diseases;
• pregnancy