Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Class |
|---|---|
| Svineafgiftsfonden | UNKNOWN |
| DC Ingredients | UNKNOWN |
| Arla Foods | INDUSTRY |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
The purpose of the study is to obtain a better understanding of how hydrolysed porcine proteins affect the human metabolism, including the effects on muscle protein synthesis, appetite and secretion of gastrointestinal hormones.
It is hypothesised that hydrolysed porcine proteins will stimulate muscle protein synthesis, affect appetite and the secretion of gastrointestinal hormones similar to hydrolysed whey protein.
Previous data show that proteins enhance satiety, decrease hunger and decrease energy intake. In addition, amino acids from dietary protein act as building blocks for de novo protein synthesis and consumption of dietary protein therefore stimulates protein synthesis. Proteins from different sources differ in amino acid composition and rate of absorption. Previously, studies on the effects of proteins on especially muscle protein synthesis has focused on milk proteins, whey and casein as animal sources, but other protein sources high in essential and branched-chain amino acids may also have beneficial effects on muscle protein synthesis and appetite. Thus, in this study the effects of two hydrolysed porcine proteins (one from porcine blood and one from porcine muscle) on muscle protein synthesis, appetite and secretion of gastrointestinal hormones will be compared with the effects of hydrolysed whey protein. These porcine proteins have never previously been tested and it is hypothesised that they may induce similar effects as hydrolysed whey protein due to the high contents of essential and branched-chain amino acids.
The study will be conducted as a randomised, 3-way, cross-over study. It consists of three visits separated by at least two weeks. It is expected that 18 normal-weight, young men will complete the study. They will be randomised to the order of the three proteins; hydrolysed porcine protein from blood (HPB), hydrolysed porcine protein from muscle (HPM) and hydrolysed whey protein (HW). At each visit the effects on muscle protein synthesis, appetite and secretion of gastrointestinal hormones will be measured/assessed. Muscle protein synthesis will be measured after consumption of a low (15 g) and a high (30 g) dose of protein and appetite and secretion of gastrointestinal hormones will be measured after the high protein dose (30 g). The effect on muscle protein synthesis will be measured by a flood-primed continuous infusion of labelled (Ring13C6) phenylalanine, muscle biopsies and blood samples. The effects on appetite will be assessed by visual analogue scales, an ad libitum meal and blood samples.
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Hydrolysed porcine protein from blood | Experimental | Dietary intervention with hydrolysed porcine protein from blood |
|
| Hydrolysed porcine protein from muscle | Experimental | Dietary intervention with hydrolysed porcine protein from muscle |
|
| Hydrolysed whey protein | Experimental | Dietary intervention with hydrolysed whey protein |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Hydrolysed porcine protein from blood | Dietary Supplement | Effects of hydrolysed porcine proteins on muscle protein synthesis and appetite |
|
| Measure | Description | Time Frame |
|---|---|---|
| Change in Muscle protein synthesis (%FSR) | 3 biopsies are taken at time 0, 150 min and 310 min at each visit | |
| Change in Muscle protein synthesis (%FSR) | 13 blood samples are taken over 310 min |
| Measure | Description | Time Frame |
|---|---|---|
| Visual analogue scales (10 cm) | Subjective appetite | Assessed at time 0, 160, 175, 200, 220, 250, 280, 310, 345 min at each of the three test days |
| Appetite - ad libitum energy intake | At 325 min an ad libitum meal of spaghetti bolognese is served, and the total energy intake is recorded |
| Measure | Description | Time Frame |
|---|---|---|
| Urate in urine and blood | Explorative parameter | Blood samples are taken at 0, 20, 40, 60, 90, 120, 150, 180, 200, 220, 250, 280 and 310 min at each of the three test days. Urine is collected over 310 min at each test day |
| Muscle protein breakdown, gene expression |
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Arne Astrup, DMSc | University of Copenhagen | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Department of Nutrition, Exercise and Sports | Frederiksberg | 1958 | Denmark |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Hydrolysed porcine protein from muscle | Dietary Supplement | Effects of hydrolysed porcine proteins on muscle protein synthesis and appetite |
|
| Hydrolysed whey protein | Dietary Supplement | Effects of hydrolysed porcine proteins on muscle protein synthesis and appetite |
|
| Measured once at time 325 min at each of the three test days |
| Postprandial response in glycemic control, plasma amino acids and appetite regulating hormones | Blood samples are taken at 0, 150, 180, 200, 220, 250, 280 and 310 min at each of the three test days |
Explorative parameter |
| 3 biopsies are taken at time 0, 150 min and 310 min. at each of the three test days |
| Metabolomics - urine and blood | Explorative parameter | Blood samples are taken at 0, 20, 40, 60, 90, 120, 150, 180, 200, 220, 250, 280 and 310 min at each of the three test days. Urine is collected over 310 min at each test day |