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The investigators want to understand how oral processing (chewing) of nuts affects particle size and the presence of lipid (fat) on the cut surfaces.
The main objective of the study is to:
Measure the size of nut particles that have been chewed sufficiently to be swallowed.
The secondary objective of this study is to:
Measure any changes in lipid content due to chewing and compare it to a prediction from a theoretical model.
The investigators have developed a theoretical model for determining the release of nutrients from plant foods, specifically lipid (fat) from almonds. The model has been used to calculate the amount of lipid released from chewed almonds. The model shows that only about 10% of the lipid is immediately released. The investigators require information on the particle size distribution (number of particles of each size) for other chewed nuts to calculate the amount of lipid released for other nuts. This will allow us to check the validity of our model for other foods.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Chewing of nuts | Experimental | 8 samples (4-5 g) of nuts (cashews or walnuts) on two separate visit days. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Chewing of nuts | Other | The volunteers will be asked to chew and spit 8 portions (4-5 g) of nuts (cashews or walnuts) on two separate visits. They will provide samples for particle sizing. Two portions will be used as practice samples in order to measure the number of chews. Two portions will be sieved. Two portions will be measured by laser diffraction. Two portions will be frozen for later lipid analysis. |
| Measure | Description | Time Frame |
|---|---|---|
| Particle size distribution of masticated nuts | Particle size distribution of masticated nuts as measured by laser diffraction and sieving. | At time of expectoration, up to 90 seconds after each sample ingested. |
| Measure | Description | Time Frame |
|---|---|---|
| Lipid content of masticated nuts | Lipid content of masticated nuts as measured by Soxhlet to be compared to predictions form mathematical model. | At time of expectoration, up to 90 seconds after each sample ingested. |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Terri Grassby, BSc PhD | King's College London | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Diabetes and Nutritional Sciences Division, School of Medicine, King's College London | London | SE1 9NH | United Kingdom |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 25527747 | Background | Grundy MM, Grassby T, Mandalari G, Waldron KW, Butterworth PJ, Berry SE, Ellis PR. Effect of mastication on lipid bioaccessibility of almonds in a randomized human study and its implications for digestion kinetics, metabolizable energy, and postprandial lipemia. Am J Clin Nutr. 2015 Jan;101(1):25-33. doi: 10.3945/ajcn.114.088328. Epub 2014 Nov 12. | |
| 25310222 |
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| Grassby T, Picout DR, Mandalari G, Faulks RM, Kendall CW, Rich GT, Wickham MS, Lapsley K, Ellis PR. Modelling of nutrient bioaccessibility in almond seeds based on the fracture properties of their cell walls. Food Funct. 2014 Dec;5(12):3096-106. doi: 10.1039/c4fo00659c. |