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The primary hypothesis of this study is that the higher concentration of soy protein intake will increase satiety and decrease the energy intake at the subsequent meal.
The secondary hypothesis is that the synergistic effect of soy protein and polydextrose will further increase satiety and reduce energy intake at the subsequent meal.
The third hypothesis is that the ingestion of protein and polydextrose will differentially impact on satiety-related metabolism, i.e. blood glucose, insulin, urea, plasma amino acids, gut hormones and gastric emptying.
Soy foods have been part of Asian food systems for millennia. In the past decades, both soy proteins and polydextrose have been widely used in a variety of food products. Helping consumers to feel less hungry is an approach to weight management because it has the potential to decrease inter-meal hunger and hence reduce overall energy intake. Considering the possible satiety effect of soy protein and polydextrose, inclusion of these molecules in the diet could therefore potentially reduce the energy intake and assist with weight management in order to attenuate the rise in obesity incidence globally. The study will provide data for the first time on how soy protein and soluble fiber impact on satiety and acute energy intake in those of Chinese ethnic origin. The research sets out to determine the effect of soy protein and polydextrose on satiety and food intake in Asians. It specifically attempts to comprehend the physiological mechanisms involving blood glucose, insulin, urea, plasma amino acids, gut hormones and the rate of gastric emptying of soy protein and polydextrose on appetite ratings, satiety and subsequent food intake. The study will recruit healthy young Chinese male, who will return for four test sessions on non-consecutive days. At each session, the subjects will be given a standard breakfast, followed 180 min later by a soybean curd preload and a buffet lunch 90 min after the preload. Four preloads will be tested: (1) low protein, (2) high protein, (3) low protein with polydextrose, and (4) high protein with polydextrose. Each soybean curd preload will be tested once by each subject. Blood samples from cannulated veins and fingerpricks will be collected before the preload, 15, 30, 45, 60, 75 and 90 min after the preload for the analysis of glucose, insulin, urea, plasma amino acids, and gut hormones. At the same time points as when blood samples collected, gastric emptying measurements will be carried out using ultrasound. Subjective motivational ratings for hunger, fullness, desire to eat and prospective food consumption will be measured by the use of visual analogue scales (VAS).
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Low Protein Preload | Active Comparator | The subjects consumed soybean curd preload containing low protein content. |
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| Low Protein with Polydextrose Preload | Experimental | The subjects consumed soybean curd preload containing low protein content and polydextrose. |
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| High Protein Preload | Experimental | The subjects consumed soybean curd preload containing high protein content. |
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| High Protein with Polydextrose Preload | Experimental | The subjects consumed soybean curd preload containing high protein content and polydextrose. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Soy Protein | Other | Soy protein is commonly consumed in Asian diet. |
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| Measure | Description | Time Frame |
|---|---|---|
| Food intake | Ad-libitum lunch will be provided to measure food intake | 90 min subsequent to preload consumption |
| Measure | Description | Time Frame |
|---|---|---|
| Subjective motivational ratings | Subjective motivational ratings for hunger, fullness, desire to eat and prospective food consumption was measured by the use of visual analogue scales (VAS). | VAS will be obtained before and after breakfast, 1 and 2 h after breakfast, before preload, 15, 30, 45, 60, and 75 min after the preload, and before and after lunch. |
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Inclusion Criteria:
Exclusion Criteria:
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| ID | Term |
|---|---|
| D009765 | Obesity |
| D003920 | Diabetes Mellitus |
| ID | Term |
|---|---|
| D050177 | Overweight |
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
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| ID | Term |
|---|---|
| D030262 | Soybean Proteins |
| C033375 | polydextrose |
| ID | Term |
|---|---|
| D010940 | Plant Proteins |
| D011506 | Proteins |
| D000602 | Amino Acids, Peptides, and Proteins |
| D014674 | Plant Proteins, Dietary |
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| Polydextrose | Other | Polydextrose is a randomly bonded polymer of glucose developed in the 1960s. It is widely recognized as a soluble fiber that is not digested in the upper gastrointestinal tract due to the complex structure and nature of the glycosidic bonds, but is partially fermented by the microbiota in large intestine generating short chain fatty acids. |
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| Gastric emptying rate | The rate of gastric emptying was measured by the use of ultrasound scanning. | Before the preload, and 15, 30, 45, 60, 75 and 90 min after the preload |
| Physiological Outcomes | Blood samples were obtained to analyze glucose, insulin, plasma amino acids, and gut hormones gut hormones | Before the preload, and 15, 30, 45, 60, 75 and 90 min after the preload |
| D001835 |
| Body Weight |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D004700 | Endocrine System Diseases |
| D004044 |
| Dietary Proteins |
| D005502 | Food |
| D000066888 | Diet, Food, and Nutrition |
| D010829 | Physiological Phenomena |
| D045730 | Soy Foods |
| D000067075 | Vegetable Products |
| D014675 | Vegetables |
| D019602 | Food and Beverages |