Study is not realised.

In vitro results showed a protective effect against acids for the mouthwash containing Curolox™ peptide. The goal of this in situ study is to detect the Curolox™ peptide under natural oral conditions on the tooth surface and to proof the in vitro results obtained. So far there are no effect and safety data of the Curolox™ mouthwash under natural oral conditions available.

The primary objective of the study is the detection of the Curolox™ peptide on the bovine enamel sample (proof-of-concept) under in vivo conditions after rinsing with the peptide-containing mouth wash.

Secondary Objectives

The secondary objectives of the study are:

1. safety and tolerance of the mouthwash in participants
2. identification of the duration the Curolox™ peptide is present on the bovine tooth surface under in vivo conditions
3. identification of the most effective mouthwash rinsing volume (2.5 mL or 5 mL)
4. prophylactic effect of multiple applications of the mouthwash confirmation of the prophylactic effect of the Curolox™ peptide obtained in in vitro study

Inclusion Criteria:

• Willing and able to understand and to follow the study procedures and instructions
• Willing to wear a dental appliance for 7 days
• Willing and able to attend the on-study visits
• Good oral hygiene throughout the study
• Written informed consent before participation in the study

Exclusion Criteria:

• Volunteer with an active periodontal disease or periodontal surgery < 1 year prior to study participation
• Volunteer with hypersensitive teeth or with any other (painful) oral pathology or defect
• Volunteer with an orthodontic appliance
• Volunteer undergoing a medical treatment
• Volunteer who is allergic to dental products
• Pregnant and lactating woman
• Concurrent participation in another clinical trial