Identify Bacteria Associated With Metabolic Diseases That Reside Both in Intestinal Crypts and in Blood

Identify Bacteria Associated With Metabolic Diseases That Reside Both in Intestinal Crypts and in Blood.

The investigators will compare 16sRDNA profile in cases and controls in order to identify bacteria associated with metabolic diseases that reside both in intestinal crypts and in blood.

Experimental evidence suggests that bacterial translocation from gut to tissue could be an initial step leading to diabetes. To test this hypothesis, a bacterial component that is highly conserved between different species of bacteria, the 16S rDNA gene will be analysed both in intestinal crypt and in blood in patients with type 2 diabetes, obese patients and control.

Inclusion Criteria:

• Between 35 and 75 years old
• Colonoscopy and biopsies required

Diabetes population:

• Type 2 diabetes

Obese population:

• Android obesity

Exclusion Criteria:

• Inflammatory bowel disease
• Colonic cancer
• Bariatric surgery
• Previous administration of antibiotic (within 15 days)
• Pregnancy