Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
The purpose of this study is to isolate genotype-specific DBP/Gc from homozygous human volunteers and use these purified proteins to cross-validate existing or novel assays for this serum protein and to evaluate the binding characteristics of these variants of DBP/Gc for different vitamin D metabolites.
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| DBP/Gc1f-1f genotype | Human volunteers homozygous for the 1f-1f (fast) genotype of DBP. |
| |
| DBP/Gc1s-1s genotype | Human volunteers homozygous for the 1s-1s (slow) genotype of DBP. |
| |
| DBP/Gc 2-2 genotype | Human volunteers homozygous for the 2-2 genotype of DBP. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Blood draw | Other | Sampling of 100-150 ml of blood |
|
| Measure | Description | Time Frame |
|---|---|---|
| Vitamin D binding protein binding characteristics (affinity constant) | In vitro characterization | single blood draw at time of consultation, an expected average 4 years after curative treatment for breast cancer |
| Measure | Description | Time Frame |
|---|---|---|
| Correlation coefficient (r) of different DBP assays | In vitro characterization | single blood draw at time of consultation, an expected average 4 years after curative treatment for breast cancer |
Not provided
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Not provided
Patients with known genotype of DBP/Gc, as identified in the clinical database of the Multidisciplinary Breast Centre, University Hospitals Leuven, and free of breast cancer recurrence at the time of blood sampling.
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Dirk Vanderschueren, MD PhD | UZ Leuven | Principal Investigator |
| Roger Bouillon, MD PhD | UZ Leuven | Study Chair |
| Hans Wildiers, MD PhD | UZ Leuven | Study Chair |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| UZ Leuven | Leuven | 3000 | Belgium |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 22623648 | Background | Hatse S, Lambrechts D, Verstuyf A, Smeets A, Brouwers B, Vandorpe T, Brouckaert O, Peuteman G, Laenen A, Verlinden L, Kriebitzsch C, Dieudonne AS, Paridaens R, Neven P, Christiaens MR, Bouillon R, Wildiers H. Vitamin D status at breast cancer diagnosis: correlation with tumor characteristics, disease outcome, and genetic determinants of vitamin D insufficiency. Carcinogenesis. 2012 Jul;33(7):1319-26. doi: 10.1093/carcin/bgs187. Epub 2012 May 23. |
Not provided
Not provided
Not provided
| ID | Term |
|---|---|
| D014808 | Vitamin D Deficiency |
| ID | Term |
|---|---|
| D001361 | Avitaminosis |
| D003677 | Deficiency Diseases |
| D044342 | Malnutrition |
| D009748 | Nutrition Disorders |
Not provided
Not provided
| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
Not provided
Not provided
Not provided
Not provided
Not provided
Serum will be derived from 100-150 ml of blood and used for protein purification
| D009750 |
| Nutritional and Metabolic Diseases |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |