Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Class |
|---|---|
| Agroscope Liebefeld-Posieux Research Station ALP | OTHER |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
The important role played by the GI tract microflora on the metabolic health of an individual are increasingly recognized. In this respect, the initial studies of Metchinkoff that suggested a role of fermented food (specifically yoghourts ) to modulate the gut microflora have evolved into the concept of "probiotics". Probiotics are living microorganisms that bring a benefit to the host when administered in sufficient quantity (WHO/FAO, 2001).
Here the investigators will study, in normal young male volunteers, the potential to modulate post-prandial metabolic and inflammatory responses by the administration of a yoghourt containing the probiotic Lactobacillus rhamnosus (LGG).
This is a prospective, randomized, double-blind and cross-over study designed to evaluate the potential of a probiotic (Lactobacillus rhamnosus) to modulate the post prandial metabolic and inflammatory response of normale healthy individuals.
After a wash out period during which volunteers will have to follow a diet devoid of all milk or milk-derived products, they will receive, in random order, either a daily dose of the probiotic, or a daily dose of acidified milk (control) for a total of three consecutive weeks. At the end of each of these period, they will be submitted to a day of investigation, during which their metabolic and inflammatory profile will be studied following the ingestion of a standard test meal. The potential for the probiotic to modulate the metabolic and inflammatory parameters will be evaluated.
The test meal will provide 1004Kcal under the form of 45.4g proteins (17%), 69g total fatty acids (62%), and 52.6g glucids (21%) as previously published (Schwander et al, J Nut 2014).
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Probiotic yogurt | Experimental | Daily administration, during two weeks, of two probiotic yogurts (200 mL each) |
|
| Acidified milk | Active Comparator | Daily administration, over two consecutive weeks, of an isocaloric dose (compared to probiotics) of unfermented acidified milk |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Probiotic yogurt | Dietary Supplement | Probiotic yogurt (fermented milk with Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus- YoFlex FD-DVS YC-X11 Fr, with the addition of Lactobacillus rhamnosus GG (LGG)- Culture Collection, University of Göteborg, Sweden CCUG 34291). |
| Measure | Description | Time Frame |
|---|---|---|
| Metabolomic analyses | Kinetics of metabolomic changes in blood during the 6 hours after meal ingestion, in presence of yogurt or placebo | 6 hours |
| Measure | Description | Time Frame |
|---|---|---|
| Glucose | Kinetics of glucose during the 6 hours after meal ingestion, in presence of yogurt or placebo | 6 hours |
| Insulin | Kinetics of insulin during the 6 hours after meal ingestion, in presence of yogurt or placebo |
| Measure | Description | Time Frame |
|---|---|---|
| Transcriptomic analyses | Kinetics of gene expression in white blood cells during the 6 hours after meal ingestion, in presence of yogurt or placebo | 6 hours |
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Francois P Pralong, MD | Centre Hospitalier Universitaire Vaudois | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Clinical research center, Lausanne University hospital | Lausanne | Canton of Vaud | 1011 | Switzerland |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 30721917 | Derived | Vionnet N, Munger LH, Freiburghaus C, Burton KJ, Pimentel G, Pralong FP, Badertscher R, Vergeres G. Assessment of lactase activity in humans by measurement of galactitol and galactonate in serum and urine after milk intake. Am J Clin Nutr. 2019 Feb 1;109(2):470-477. doi: 10.1093/ajcn/nqy296. | |
| 29788433 | Derived |
Not provided
Not provided
Not provided
| ID | Term |
|---|---|
| D007249 | Inflammation |
| D050171 | Dyslipidemias |
| ID | Term |
|---|---|
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D052439 | Lipid Metabolism Disorders |
| D008659 | Metabolic Diseases |
Not provided
Not provided
| ID | Term |
|---|---|
| D008892 | Milk |
| ID | Term |
|---|---|
| D001628 | Beverages |
| D000066888 | Diet, Food, and Nutrition |
| D010829 | Physiological Phenomena |
| D003611 | Dairy Products |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
|
|
| Acidified milk | Dietary Supplement | Milk will consist in an isocaloric supplement (compared to probiotic yogurt) of unfermented milk |
|
|
| 6 hours |
| Glucagon like polypeptide-1 | Kinetics of GLP-1 during the 6 hours after meal ingestion, in presence of yogurt or placebo | 6 hours |
| Gastric inhibitory polypeptide | Kinetics of GIP during the 6 hours after meal ingestion, in presence of yogurt or placebo | 6 hours |
| Total cholesterol | Kinetics of total cholesterol during the 6 hours after meal ingestion, in presence of yogurt or placebo | 6 hours |
| LDL-cholesterol | Kinetics of LDL-cholesterol during the 6 hours after meal ingestion, in presence of yogurt or placebo | 6 hours |
| HDL-cholesterol | Kinetics of HDL-cholesterol during the 6 hours after meal ingestion, in presence of yogurt or placebo | 6 hours |
| Non esterified fatty acids | Kinetics of NEFAs during the 6 hours after meal ingestion, in presence of yogurt or placebo | 6 hours |
| Triglycerides | Kinetics of triglycerides during the 6 hours after meal ingestion, in presence of yogurt or placebo | 6 hours |
| Interleukin-6 | Kinetics of IL-6 will be measured in blood of volunteers after the administration of yogurt or placebo | 6 hours |
| Chemokine CCK2 | Kinetics of CCK2 will be measured in blood of volunteers after the administration of yogurt or placebo | 6 hours |
| Chemokine CCK5 | Kinetics of CCK5 will be measured in blood of volunteers after the administration of yogurt or placebo | 6 hours |
| Tunour necrosis factor alpha | Kinetics of TNF-a will be measured in blood of volunteers after the administration of yogurt or placebo | 6 hours |
| Lipo-poly-saccharide | Kinetics of LPS will be measured in blood of volunteers after the administration of yogurt or placebo | 6 hours |
| Pimentel G, Burton KJ, von Ah U, Butikofer U, Pralong FP, Vionnet N, Portmann R, Vergeres G. Metabolic Footprinting of Fermented Milk Consumption in Serum of Healthy Men. J Nutr. 2018 Jun 1;148(6):851-860. doi: 10.1093/jn/nxy053. |
| D009750 | Nutritional and Metabolic Diseases |
| D005502 |
| Food |
| D019602 | Food and Beverages |