DEPORRA-CoProst: Assessment of New Fluorescence Imagery Techniques Using Prostate Chips From Transurethral Resections

Assessment of New Fluorescence Imagery Techniques Using Prostate Chips From Transurethral Resections.

The aim of this study is divided in 4 different steps:

• the first step has two different purpose: assess the impact of the storage medium of fresh prostatic chips on fluorescence signal and adjust the entire chain (immunolabelling,counter-stain and imaging),

• the second step is the adaptation of immunolabelling protocol on histopathology slides, using fresh prostatic tissue,

• the third step is to validate the use of:

  1. the medical device created by the FEMTO-ST institute use for the detection of fluorescence signal on fresh tissue,
  2. the Light-CT scanner use for tissue structural observation.

• the four step is to check the preservation of morphological structure of tissue under the effect of laser excitation from the medical device.

Inclusion Criteria:

• Male patient over 18 years old.

• Patient scheduled for trans-urethral resection of prostate with:

  1. prostate specific antigen (PSA) blood level ≤ 4 ng/ml and age > 80 years old
  2. a suspected prostate cancer after a digital rectal examination (DRE) and a PSA blood level > 50 ng/ml or an existing prostate cancer

• Patient affiliated to social security or beneficiary of such a regime.

Exclusion Criteria:

• Protected patient referred to in Articles L1121-6 to L1121-8 of the Code of Public Health