Results of 5 first patients show that chips' cauterization interferes the signal

The aim of this study is divided in 4 different steps:

• the first step has two different purpose: assess the impact of the storage medium of fresh prostatic chips on fluorescence signal and adjust the entire chain (immunolabelling,counter-stain and imaging),

• the second step is the adaptation of immunolabelling protocol on histopathology slides, using fresh prostatic tissue,

• the third step is to validate the use of:

  1. the medical device created by the FEMTO-ST institute use for the detection of fluorescence signal on fresh tissue,
  2. the Light-CT scanner use for tissue structural observation.

• the four step is to check the preservation of morphological structure of tissue under the effect of laser excitation from the medical device.

Inclusion Criteria:

• Male patient over 18 years old.

• Patient scheduled for trans-urethral resection of prostate with:

  1. prostate specific antigen (PSA) blood level ≤ 4 ng/ml and age > 80 years old
  2. a suspected prostate cancer after a digital rectal examination (DRE) and a PSA blood level > 50 ng/ml or an existing prostate cancer

• Patient affiliated to social security or beneficiary of such a regime.

Exclusion Criteria:

• Protected patient referred to in Articles L1121-6 to L1121-8 of the Code of Public Health