PORH and Response to Cold in Raynaud's Phenomenon.

Rôle Des Acides Epoxy-eicosatriénoïques et du Monoxyde d'Azote Dans l'hyperhémie Post-occlusive cutanée Digitale et la réponse cutanée au Froid Dans le phénomène de Raynaud Primaire

The main objective of the study is to determine if implication of epoxy-eicosatriénoïc acids (EETs) and NO during cutaneous post-occlusive hyperemia differs between patients with Raynaud phenomena and healthy volunteers, by studying hyperaemic postocclusive response after microinjection of fluconazole and L-NMMA at the dorsal side of the fingers.

The main objective of the study is to determine if implication of epoxy-eicosatriénoïques acids (EETs) and NO during cutaneous post-occlusive hyperemia differs between patients with Raynaud phenomena and healthy volunteers, by studying hyperaemic postocclusive response after microinjection of fluconazole and L-NMMA at the dorsal side of the fingers.

Healthy controls and patients will undergo 3 visits

Inclusion Criteria:

• Neither raynaud's phenomenon or chronic disease for healthy volunteers
• Raynaud's phenomenon without connective tissue disease for patients in the group "Raynaud"

Exclusion Criteria:

• History of axillary dissection , trauma or surgery
• History of thromboembolic disease or thrombophilia
• Minor or law-protected major
• Exclusion period in another study
• No affiliation to medicare
• Pregnant, parturient or breasting woman
• Concomitant serious disease: progressive cancer, liver failure, history of myocardial infarction less than 5 years, angor
• Smoking in the 6 last months
• Person deprived of liberty by a legal or administrative decision, person under legal protection
• Maximal annual indemnification reached.