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| ID | Type | Description | Link |
|---|---|---|---|
| 12G1714N | Other Grant/Funding Number | Fund for Scientific Research Flanders |
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| Name | Class |
|---|---|
| Fund for Scientific Research, Flanders, Belgium | OTHER |
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In this project proposal, the investigators will investigate the genetic alterations of Hepatitis B Virus (HBV) strains circulating in Belgian patients who developed end stage liver disease. Additionally, the investigators will compare and link these data sets with three genetic factors involved in immune system response.
This project proposes to identify and characterize the genetic alterations associated with intra-host evolution of HBV from a chronically infection status to an end-stage liver disease status.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Chronic Hepatitis B | Diagnosis of Hepatitis B related liver disease in a pre-advanced stage | ||
| End stage liver disease | Diagnosis of advanced liver disease related to Hepatitis B |
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| Measure | Description | Time Frame |
|---|---|---|
| genetic variants of Hepatitis B | Virus variants will be identified by amino acid or nucleotide variations (insertions or deletions) in different Open Readind Frames (ORFs) of the HBV genome by using next-generation sequencing. Comparison of virus variants within one patient Comparison of virus variants between patients with end stage liver disease and patients with a chronic Hepatitis B infection. Determination of the viral load by quantitative Polymerase Chain Reaction (PCR). | 6 months |
| Measure | Description | Time Frame |
|---|---|---|
| genetic variation in host-specific immune markers | Amplification of HLA-A, HLA-B, HLA-C class I and HLA class II using PCR methods and next-generation sequencing Amplification of KIR genes: 2DS1, 2DS2, 2DS3, 2DS4, 2DS5, 3DS1 and 1D, 2DL1 2DL2, 2DL3, 2DL5 and 3DL1 using PCR methods and next-generation sequencing Amplification of SNPs in TNF alfa, TGF beta1 and IFN-R using PCR methods and next-generation sequencing |
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Inclusion Criteria:
Study group:
Control group:
Exclusion Criteria:
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Patients with liver disease related to Hepatitis B in a pre-advanced (chronic infection) and advanced stage (end-stage liver diseases) treated at the Hepatology Department of University Hospital of Leuven
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| Name | Affiliation | Role |
|---|---|---|
| MahmoudReza Pourkarim, PhD | UZ Leuven campus Gasthuisberg, Herestraat 49. 3000 Leuven, Belgium | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University Hospitals Leuven campus Gasthuisberg | Leuven | Flemish Brabant | 3000 | Belgium |
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| ID | Term |
|---|---|
| D006509 | Hepatitis B |
| D019694 | Hepatitis B, Chronic |
| D058625 | End Stage Liver Disease |
| D006528 | Carcinoma, Hepatocellular |
| ID | Term |
|---|---|
| D000086982 | Blood-Borne Infections |
| D003141 | Communicable Diseases |
| D007239 | Infections |
| D018347 | Hepadnaviridae Infections |
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serum whole blood
| at day of enrollment |
| D004266 |
| DNA Virus Infections |
| D014777 | Virus Diseases |
| D006525 | Hepatitis, Viral, Human |
| D006505 | Hepatitis |
| D008107 | Liver Diseases |
| D004066 | Digestive System Diseases |
| D006521 | Hepatitis, Chronic |
| D002908 | Chronic Disease |
| D020969 | Disease Attributes |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D017093 | Liver Failure |
| D048550 | Hepatic Insufficiency |
| D000230 | Adenocarcinoma |
| D002277 | Carcinoma |
| D009375 | Neoplasms, Glandular and Epithelial |
| D009370 | Neoplasms by Histologic Type |
| D009369 | Neoplasms |
| D008113 | Liver Neoplasms |
| D004067 | Digestive System Neoplasms |
| D009371 | Neoplasms by Site |