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The investigators hypothesis:
Presence of anti-GGT (antibody against GGT) indicates H. pylori infection.
Subjects will be requested to provide 1-5g of their solid/semi-solid stool sample, to be collected within 24 hours prior to their scheduled visit for urea breath test. Patients should not have been treated for H. pylori prior to stool collection. Stool samples will be tested using ELISA for the presence of the H. pylori GGT antigen using monoclonal antibodies against GGT. Stool samples will also be tested for the presence of H. pylori DNA using PCR and Western blot analysis respectively.
Presence of the GGT antigen in stool will be compared between H. pylori-positive and H. pylori-negative patients. Rapid urease test results will be used as the gold standard of H. pylori diagnosis. The sensitivity and specificity of the stool antigen test will be established.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Helicobactor Pylori Infection | Comparison on the presence/absence of GGT antigen in the stool will be compared between H. pylori-positive and H. pylori-negative subjects. Rapid urease test result from the respective patients will be used as the golden standard. Should the GGT antigen in stool samples show promise in distinguishing between H. pylori-infected and uninfected individuals, sensitivity and specificity of the stool antigen test can then be established. |
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| Measure | Description | Time Frame |
|---|---|---|
| Stool anti-GGT for the diagnosis of Helicobactor Pylori Infection | 1-5g of stool samples will also be collected from patients recruited to this study, in a preliminary investigation to determine the sensitivity and specificity of a stool H.pylori antigen test. Rapid urese test is the gold standard. | 1 month after stool samples collected |
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Inclusion Criteria:
Exclusion Criteria:
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A total of 100 patients seen in Gastro clinic will be recruited for this study. Patient should 21 years and above and agreeable for study. Patient should not have been treated for H. pylori prior to stool collection.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Lee Guan Lim | Contact | 67795555 |
| Name | Affiliation | Role |
|---|---|---|
| Lee Guan Lim | NUHS | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Nuhs Umc/Udc | Recruiting | Singapore | Singapore | Singapore |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 20347814 | Background | Gong M, Ling SS, Lui SY, Yeoh KG, Ho B. Helicobacter pylori gamma-glutamyl transpeptidase is a pathogenic factor in the development of peptic ulcer disease. Gastroenterology. 2010 Aug;139(2):564-73. doi: 10.1053/j.gastro.2010.03.050. Epub 2010 Mar 27. |
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Stool samples(solid/semi-solid) between 1-5 g will be collected and tested using ELISA for the presence of the H. pylori GGT antigen using monoclonal antibodies against GGT.