RNAseq Analysis of Microbial Gene Expression in Necrotizing Soft Tissue Infections

Hypothesis: We hypothesize that RNAseq will allow us to learn more about the causative agents of necrotizing soft tissue infections (NSTI) and risk factors involved in acquiring these devastating infections

Tissue biopsies will be obtained from consented patients admitted with NSTI. Biopsies will be used for standard clinical analysis of bacterial species present or stored for later RNA sequencing. RNA sequencing will identify bacteria that are present within the infection site that may not be detected using standard culture techniques as well as reveal bacterial gene expression profiles within the NSTI site. Medical charts will also be reviewed for basic patient information as well as wound care management practices. We hope to identify bacterial species commonly present in these types of infections as well as risk factors predisposing individuals to NSTI's.

Inclusion Criteria:

• Patient or LAR agrees to the procedure and signs informed consent form
• Patient is at least 18 yrs of age
• Patient has been diagnosed with an NSTI

Exclusion Criteria:

• Patient not willing to undergo additional tissue biopsies for RNA sequencing analysis