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| ID | Type | Description | Link |
|---|---|---|---|
| NL 44735.029.13 | Other Identifier | The Central Committee on Research Involving Human Subjects (CCMO) | |
| 91613082 | Other Grant/Funding Number | ZonMw |
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The aim of this study is to investigate the effects of genetic and environmental risk factors on central nervous system (CNS) reward and satiety circuits in the etiology of obesity. The investigators will also investigate to what extent the alterations in CNS reward and satiety circuits are a cause or a consequence of the development of obesity.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Monozygotic twin pairs | 15 monozygotic twin pairs discordant for obesity | ||
| Genetic predisposition for obesity | 15 obese and 15 non-obese individuals with a high genotype obesity risk score and 15 obese and 15 non-obese individuals with a low genotype obesity risk score. Genotype obesity risk score will be based on genome wide association single-nucleotide polymorphisms (SNP's) associated with obesity. |
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| Measure | Description | Time Frame |
|---|---|---|
| The difference in neuronal activity in CNS reward and satiety circuits as represented by BOLD functional magnetic resonance imaging (fMRI) signal change from baseline (%) in response to food-related stimuli within obesity discordant MZ twin pairs. | Baseline (one measurement) | |
| The difference in neuronal activity in CNS reward and satiety circuits as represented by BOLD fMRI signal change from baseline (%) in response to food-related stimuli between individuals at high verses low genetic obesity risk. | Baseline (one measurement) | |
| The difference in neuronal activity in CNS reward and satiety circuits as represented by BOLD fMRI signal change from baseline (%) in response to food-related stimuli between lean and obese subjects with either high or low genetic obesity risk. | Baseline (one measurement) |
| Measure | Description | Time Frame |
|---|---|---|
| The difference within obesity-discordant monozygotic (MZ) twin pairs regarding dietary intake | Dietary intake will be measured as quantitative (kcal/day) and qualitative (energy density (kcal/kg) and macronutrient composition (%)) dietary intake using A) a choice lunch buffet on the visit day and B) the 24 hours recall method on two week days and one weekend day at home. | Baseline (one measurement) |
| Measure | Description | Time Frame |
|---|---|---|
| The difference within obesity-discordant monozygotic (MZ) twin pairs regarding gut microbiota composition | Gut microbiota composition (ratio between various gut microbiota species) will be assessed by identifying microbial phylotypes in a feces sample using 16S ribosomal RNA molecule-based approaches of diagnostic analysis. Function of gut microbiota will be investigated using metaproteomics analysis techniques that include mass-spectroscopy methodologies. Please note: this outcome measure is conditional to available budget. |
Inclusion Criteria:
Exclusion Criteria:
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Subjects will be recruited from the Netherlands Twin Registry (NTR). All subjects are voluntary participants and have received surveys on lifestyle and health every 2 years. In addition, genome wide data are available in a large number of these individuals.
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| VU University Medical Center | Amsterdam | 1081HV | Netherlands |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 28597337 | Derived | Doornweerd S, De Geus EJ, Barkhof F, Van Bloemendaal L, Boomsma DI, Van Dongen J, Drent ML, Willemsen G, Veltman DJ, IJzerman RG. Brain reward responses to food stimuli among female monozygotic twins discordant for BMI. Brain Imaging Behav. 2018 Jun;12(3):718-727. doi: 10.1007/s11682-017-9711-1. | |
| 27106364 | Derived |
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| ID | Term |
|---|---|
| D009765 | Obesity |
| D050177 | Overweight |
| ID | Term |
|---|---|
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
| D001835 | Body Weight |
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| The difference within obesity-discordant monozygotic (MZ) twin pairs regarding physical activity level | Physical activity level will be measured as metabolic equivalent of task (METs)-hours per week using seven-day ActiGraph triaxial accelerometry at home. | Baseline (one measurement) |
| The difference within obesity-discordant monozygotic (MZ) twin pairs regarding basal metabolic rate | Basal metabolic rate will be measured in kcal/day using oxygen consumption and carbon dioxide production measured by indirect calorimetry using a ventilated hood system. | Baseline (one measurement) |
| The difference within obesity-discordant monozygotic (MZ) twin pairs regarding fasting circulating biomarker levels | Fasting circulating biomarker levels (glucose (mmol/l), HbA1c (mmol/mol), insulin (pmol/l), C-peptide (nmol/l), glucagon (pmol/l), triglycerides (mmol/l), HDL-cholesterol (mmol/l) and LDL-cholesterol (mmol/l)) will be measured in a fasting blood sample. | Baseline (one measurement) |
| The difference within obesity-discordant monozygotic (MZ) twin pairs regarding autonomic nervous system balance | Autonomic nervous system balance will be assessment based on measurements of A) heart rate variability (HRV; calculated as root mean square of successive R-R interval differences (RMSSD) in ms2); B) respiratory rate (RR; breaths/min) and C) respiratory sinus arrhythmia (RSA; ms) in resting conditions and in response to a mental arithmetic task using an electro- and impedance cardiogram. | Baseline (one measurement) |
| The difference between individuals at high versus those at low genetic obesity risk regarding dietary intake | Dietary intake will be measured as quantitative (kcal/day) and qualitative (energy density (kcal/kg) and macronutrient composition (%)) dietary intake using A) a choice lunch buffet on the visit day and B) the 24 hours recall method on two week days and one weekend day at home. | Baseline (one measurement) |
| The difference between individuals at high versus those at low genetic obesity risk regarding physical activity level | Physical activity level will be measured as metabolic equivalent of task (METs)-hours per week using seven-day ActiGraph triaxial accelerometry at home. | Baseline (one measurement) |
| The difference between individuals at high versus those at low genetic obesity risk regarding basal metabolic rate | Basal metabolic rate will be measured in kcal/day using oxygen consumption and carbon dioxide production measured by indirect calorimetry using a ventilated hood system. | Baseline (one measurement) |
| The difference between individuals at high versus those at low genetic obesity risk regarding fasting circulating biomarker levels | Fasting circulating biomarker levels (glucose (mmol/l), HbA1c (mmol/mol), insulin (pmol/l), C-peptide (nmol/l), glucagon (pmol/l), triglycerides (mmol/l), HDL-cholesterol (mmol/l) and LDL-cholesterol (mmol/l)) will be measured in a fasting blood sample. | Baseline (one measurement) |
| The difference between individuals at high versus those at low genetic obesity risk regarding autonomic nervous system balance | Autonomic nervous system balance will be assessment based on measurements of A) heart rate variability (HRV; calculated as root mean square of successive R-R interval differences (RMSSD) in ms2); B) respiratory rate (RR; breaths/min) and C) respiratory sinus arrhythmia (RSA; ms) in resting conditions and in response to a mental arithmetic task using an electro- and impedance cardiogram. | Baseline (one measurement) |
| Baseline (one measurement) |
| The difference within obesity-discordant monozygotic (MZ) twin pairs regarding epigenetic changes | Epigenetic changes will be assessed by measuring global DNA methylation (%) and locus specific DNA methylation (%) in regions of selected loci using a mass spectrometry-based method. Loci will be selected on the basis of their previously shown features of epigenetic regulation and their involvement in cardiovascular and metabolic disease. Please note: this outcome measure is conditional to available budget. | Baseline (one measurement) |
| The difference between individuals at high versus those at low genetic obesity risk regarding gut microbiota composition | Gut microbiota composition (ratio between various gut microbiota species) will be assessed by identifying microbial phylotypes in a feces sample using 16S ribosomal RNA molecule-based approaches of diagnostic analysis. Function of gut microbiota will be investigated using metaproteomics analysis techniques that include mass-spectroscopy methodologies. Please note: this outcome measure is conditional to available budget. | Baseline (one measurement) |
| The difference between individuals at high versus those at low genetic obesity risk regarding epigenetic changes | Epigenetic changes will be assessed by measuring global DNA methylation (%) and locus specific DNA methylation (%) in regions of selected loci using a mass spectrometry-based method. Loci will be selected on the basis of their previously shown features of epigenetic regulation and their involvement in cardiovascular and metabolic disease. Please note: this outcome measure is conditional to available budget. | Baseline (one measurement) |
| Doornweerd S, IJzerman RG, van der Eijk L, Neter JE, van Dongen J, van der Ploeg HP, de Geus EJ. Physical activity and dietary intake in BMI discordant identical twins. Obesity (Silver Spring). 2016 Jun;24(6):1349-55. doi: 10.1002/oby.21475. Epub 2016 Apr 23. |
| D012816 |
| Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |