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To investigate the effect of low oxygen tension during in vitro culture in terms of embryo quality and outcomes of pregnancy in vitro fertilization/ Intracytoplasmic sperm injection
The oocytes will be divided into two parts(two groups) form one patient. The two groups of oocytes will be cultured seperately in the incubator with the concentrations of 5% CO2,6%O2,89% N2(low oxygen concentration) verse 6% CO2,20% O2,74% N2(in atmosphere).
A series of observational measurement(embryo quality and outcomes of pregnancy) will be performed.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| 5% oxygen | Experimental | the oocytes and embryos are cultured in 5% oxygen concentration after oocytes retrieval until Day 6 (counting from fertilization) |
|
| 20% oxygen | No Intervention | the oocytes and embryos are cultured in 20% oxygen concentration (in atmosphere) after oocytes retrieval until Day 6(counting from fertilization) |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| 5% oxygen concentration | Other | the oocytes and embryos are cultured in the incubator with concentrations of 6% CO2,5%O2, and 89% N2 |
|
| Measure | Description | Time Frame |
|---|---|---|
| embryo quality | Embryo morphology was assessed on day 2, day 3 day 4 day 5 day and 6 by considering the number of blastomeres, symmetry and granularity of blastomeres, type and percentage of fragmentation, presence of multinucleated blastomeres, and degree of compaction. Gardner's blastocyst scoring system is used for blastocyst assessment | up to 6 days after fertilization |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Juanzi Shi, Doctor | Contact | +86-029-87219120 | shijuanzi123@126.com |
| Name | Affiliation | Role |
|---|---|---|
| Wenhao Shi, Master | Maternal and Children Health Care Hospital | Study Director |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Maternal and Child Health Care Hospital | Recruiting | Xi'an | Shaanxi | 710003 | China |
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