The Effect of Low Oxygen Tension on the Early Development of Human Embryo in IVF/ICSI

The Effect of Low Oxygen Tension on the Early Development of Human Embryo in IVF/ICSI

To investigate the effect of low oxygen tension during in vitro culture in terms of embryo quality and outcomes of pregnancy in vitro fertilization/ Intracytoplasmic sperm injection

The oocytes will be divided into two parts(two groups) form one patient. The two groups of oocytes will be cultured seperately in the incubator with the concentrations of 5% CO2,6%O2,89% N2(low oxygen concentration) verse 6% CO2,20% O2,74% N2(in atmosphere).

A series of observational measurement(embryo quality and outcomes of pregnancy) will be performed.

Inclusion Criteria:

• Fresh cycles of in vitro fertilization/intracytoplasmic sperm injection and emrbyo transfer (IVF/ICSI-ET)
• ≥ 10 oocytes after oocyte retrieval

Exclusion Criteria:

• ≥ 3 transfer or oocyte retrieval cycles
• PGD/PGS cycles
• half-ICSI/rescure ICSI
• fluid in the uterine cavity, abnormal endometrium, uterine abnormalities,pelvic inflammation
• acute maternal problems.
• One or both spouses have an abnormal karyotype (including polymorphism)