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In our centre, the implantation rate of frozen blastocysts (cryopreserved using vitrification)was 20,3% (Van Landuyt et al. 2011). This implantation rate is lower when compared to data from other observational studies. Retrospective studies have shown that artificial shrinkage of the blastocoelic cavity (also called collapse) and dehydration of the blastocyst just before vitrification can have a positive effect on the survival after warming. This collapse can be induced by using a laser. The study of Iwayama et al. 2010 has shown a similar survival rate but an increase in the implantation rate of collapsed blastocysts compared to non-collapsed blastocysts.
The aim of the study is to investigate the effect of artificial shrinkage by laser-induced collapse on the implantation potential of vitrified-warmed day 5 or day 6 blastocyst stage embryos in a prospective randomised controlled trial.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Collapse | Experimental | This arm includes the patients where laser-induced collapse (=artificial shrinkage) of all blastocysts is performed before vitrification |
|
| control | No Intervention | This control arm includes the patients of which the blastocysts are vitrified without performing collapse |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Collapse | Procedure | inducing collapse (= artificial shrinkage) by applying 1 or 2 laser pulses between trophectoderm cells of the blastocyst to be vitrified |
|
| Measure | Description | Time Frame |
|---|---|---|
| implantation rate of the embryo in the first frozen transfer cycle | patients are enrolled in the study at oocyte retrieval. When patient is not pregnant after fresh embryo transfer, she comes back to use the frozen embryos at least one month after the initial inclusion. After the frozen embryo transfer, poshCG is measured 14 days after transfer. Approximately 10 weeks after transfer, implantation rate of the frozen embryos is assessed. This is the number of intra-uterine gestational sacs seen on ultrasound on the number of embryos transferred | approximately 10 weeks after the first frozen embryo transfer |
| Measure | Description | Time Frame |
|---|---|---|
| survival rate of warmed blastocysts | this is the morphological assessment of survival of the blastocyst after warming | 0-30 minutes |
| implantation rate per warmed blastocyst | implantation rate of the transferred embryo per embryo initially warmed in that frozen transfer cycle. |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Lisbet Van Landuyt, MSc | Centre for Reproductive Medicine | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Centre for Reproductive Medicine | Brussels | 1090 | Belgium |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 26364080 | Derived | Van Landuyt L, Polyzos NP, De Munck N, Blockeel C, Van de Velde H, Verheyen G. A prospective randomized controlled trial investigating the effect of artificial shrinkage (collapse) on the implantation potential of vitrified blastocysts. Hum Reprod. 2015 Nov;30(11):2509-18. doi: 10.1093/humrep/dev218. Epub 2015 Sep 12. |
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| ID | Term |
|---|---|
| D014474 | Unconsciousness |
| ID | Term |
|---|---|
| D003244 | Consciousness Disorders |
| D019954 | Neurobehavioral Manifestations |
| D009461 | Neurologic Manifestations |
| D009422 | Nervous System Diseases |
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|
| approximately 10 weeks after first frozen transfer |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |