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In view of the hormonal changes induced by both sleep deprivation and obstructive sleep apnea syndrome (OSA), and the importance of maintaining these hormones in reproductive processes. The present study aims to evaluate the effects of the OSA or sleep deprivation (total or selective REM sleep for 48h) on reproductive function in adult men with normal body mass index.
In this study will be recruited volunteers 25-50 years of age with a body mass index (BMI) 20-25 kg/m2 (normal) that after performing polysomnography, will be distributed in the following groups: healthy or diagnosed with obstructive sleep apnea syndrome (OSA). Volunteers without OSA will be submitted to total sleep deprivation (SD) or selective REM sleep deprivation for 48 hours. After SD, these volunteers will be allowed to sleep for 48 hours (period rebound). Blood samples will be taken for hormonal analysis (testosterone, luteinizing hormone, follicle-stimulating hormone, progesterone and cortisol)and gene expression. The semen collection for evaluation of sperm count (concentration, viability and sperm morphology) in three different periods, baseline collection, after SD protocols and the end of rebound period. Individuals diagnosed with OSA also have three collections of blood and semen samples for analyzes.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Total sleep deprivation | Healthy volunteers will be submitted to total sleep deprivation (TSD) for 48 hours. |
| |
| moderate to severe OSA | Patients diagnosed with moderate to severe obstructive sleep apnea syndrome (OSA) with normal or overweight body mass index (BMI), will be recruited. | ||
| REM sleep deprivation | Healthy volunteers will be submitted to selective REM sleep deprivation (REMSD) for 48 hours. |
| |
| Control | The control group will be subjects with an apnea hypopnea index <5/h. |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Sleep deprivation | Behavioral | Volunteers will be submitted to total sleep deprivation (SD) or selective REM sleep deprivation for 48 hours. After SD, these volunteers will be allowed to sleep for 48 hours (period rebound). |
| Measure | Description | Time Frame |
|---|---|---|
| Change in sperm analysis | - Semen collection (concentration, viability and sperm morphology) | change from baseline, sleep deprivations and rebound period. Participants will be followed for the duration of laboratory stay, an expected average of 7 days |
| Measure | Description | Time Frame |
|---|---|---|
| change hormonal evaluation | - Hormonal analysis (testosterone, luteinizing hormone, follicle-stimulating hormone, progesterone and cortisol) | change from baseline, sleep deprivations and rebound period. Participants will be followed for the duration of laboratory stay, an expected average of 7 days |
| Measure | Description | Time Frame |
|---|---|---|
| Sleep patterns of the study population | - Polysomnography | Change from baseline, sleep deprivations and rebound period. Participants will be followed for the duration of laboratory stay, an expected average of 7 days |
| Change gene expression |
Inclusion Criteria:
Exclusion Criteria:
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Tathiana A Alvarenga, PhD | Federal University of São Paulo | Principal Investigator |
| Monica L Andersen, Prof | Federal University of São Paulo | Study Director |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Universidade Federal de Sao Paulo | São Paulo | São Paulo | 04024002 | Brazil |
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| Label | URL |
|---|---|
| American Academy of Sleep Medicine | View source |
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| ID | Term |
|---|---|
| D020181 | Sleep Apnea, Obstructive |
| D018761 | Multiple Endocrine Neoplasia Type 1 |
| ID | Term |
|---|---|
| D012891 | Sleep Apnea Syndromes |
| D001049 | Apnea |
| D012120 | Respiration Disorders |
| D012140 | Respiratory Tract Diseases |
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Blood will be collected in specific tubes (PaxGene)for RNA extraction and gene expression determination.
| Change from baseline, sleep deprivations and rebound period. Participants will be followed for the duration of laboratory stay, an expected average of 7 days |
| D020919 |
| Sleep Disorders, Intrinsic |
| D020920 | Dyssomnias |
| D012893 | Sleep Wake Disorders |
| D009422 | Nervous System Diseases |
| D009377 | Multiple Endocrine Neoplasia |
| D004701 | Endocrine Gland Neoplasms |
| D009371 | Neoplasms by Site |
| D009369 | Neoplasms |
| D009378 | Neoplasms, Multiple Primary |
| D009386 | Neoplastic Syndromes, Hereditary |
| D030342 | Genetic Diseases, Inborn |
| D009358 | Congenital, Hereditary, and Neonatal Diseases and Abnormalities |
| D004700 | Endocrine System Diseases |