This study is to collect blood and urine samples to help identify and validate protein biomarkers of recovery from moderate or severe acute kidney injury (AKI).

Inclusion Criteria:a.

• Males and females 21 years of age or older
• Receiving care in an intensive care unit
• Expected to remain in the ICU for at least 48 hours after enrollment
• Use of indwelling urinary catheter as standard care at the time of enrollment
• Subject must have acute kidney injury (KDIGO stage 2 or stage 3) at the time of the first sample collection
• First sample must be collected within 36 hours of meeting KDIGO stage 2 criteria
• Written informed consent provided by patient or legally authorized representative (LAR)

Exclusion Criteria:

• Prior kidney transplantation

• Comfort-measures-only status

• Already receiving dialysis (either acute or chronic) or in imminent need of dialysis at the time of enrollment

• History of human immunodeficiency virus (HIV) or hepatitis virus (based upon available medical records) infections (NOTE: HIV or hepatitis testing need not be performed for enrollment in this study.)

• Special populations, pregnant women, prisoners or institutionalized individuals

• Patient meets any of the following:

  • Active bleeding with an anticipated need for > 4 units PRBC in a day
  • Hemoglobin < 7 g/dL
  • Any other condition that in the physician's opinion would contraindicate drawing serial blood samples for clinical study purposes

Adult ICU Patients with AKI