Treatment of Chronic Critical Limb Ischemia With G-CSF-mobilized Autologous Peripheral Blood Mononuclear Cells

Pilot Study of Treatment of Chronic Critical Limb Ischemia With G-CSF-mobilized Autologous Peripheral Blood Mononuclear Cells

The purpose of this study is to determine the safety and efficacy of G-CSF-mobilized autologous peripheral blood mononuclear cell injection to ischemic limbs of patients with critical limb ischemia.

Inclusion Criteria:

• Patients with critical limb ischemia confirmed by toe pressure, ABI, TCOM
• Levels of arterial occlusion are femoropopliteal or tibioperoneal occlusion
• Poor distal artery runoff
• Age 18-70 year

Exclusion Criteria:

• Planned for major amputation within 4 weeks
• Receive blood component within 4 weeks
• Acute myocardial infarction
• severe valvular heart disease
• renal failure
• liver failure
• Cancer
• Hypercoagulable state
• Severe infection
• Pregnancy