Bacteriophage Effects on Pseudomonas Aeruginosa

Bacteriophages Effects on Pseudomonas Aeruginosa Presents in Sputum of Cystic Fibrosis (CF) Patients

Pulmonary phage therapy to treat bacterial infections of the respiratory tract have been investigated in animals. The aim of the present study is to evaluate the efficacy of bacteriophages in infecting Pseudomonas aeruginosa (PA) strains present in sputum samples. A cocktail of 10 bacteriophages will be applied on 60 sputum samples obtained from cystic fibrosis (CF) patients during 6 hours.We will determine the bacteria and bacteriophages strains in sputum samples collected. Then the sensitivity of individual colony will be tested.

Inclusion Criteria:

• Informed consent
• Affiliated or benefit from a disease insurance regimen
• Men and women
• Aged from at least 6 years old
• Confirmed Diagnosis of cystic fibrosis based on presence of 2 mutations of CFTR gene and/or 2 positive tests of sweat chloride and/or 2 measures of pathologic difference of nasal potential associated to cystic fibrosis clinical signs.
• Patients able to produce sputum
• Pseudomonas aeruginosa Chronic infected patients

Exclusion Criteria:

• Simultaneous participation to another project on anti-infection, anti-inflammatory or modificating agents
• Subjet in exclusion period
• Law protected patient
• Realisation of sputum production is contra-indicated