The purpose of this project is to evaluate the level of mitochondrial dysfunction several patient populations: Burn, trauma, and control group of healthy volunteers.

Study hypothesis: Increased plasma concentrations of a newly discovered inflammatory mediated, called mtDNA DAMPS associated with the occurrence of multi-organ dysfunction syndrome in severly injured patients.

As the severity of a burn injury or trauma injury increase, so will systemic mitochondrial dysfunction.

Increased plasma concentrations of a newly discovered inflammatory mediated, called mtDNA DAMPS associated with the occurrence of multi-organ dysfunction syndrome in severly injured patients.

A normal healthy sub-population will be used as a control.

Inclusion Criteria:

• Burn injury must be less than 24 hours old at time of initial sampling
• Burn must be 2nd or 3rd degree and at least 10% TBSA
• ISS (injury severity score) > 15

Exclusion Criteria:

• Burn injury/trauma injury > than 24hours old at time of initial sampling
• Burn < 10% total body surface area.
• Evidence of active infection on admission in the burn subjects.
• < 19 years of age and > 70 years