Optimizing Uptake of Methylaminolevulinat With Fractional Ablative Laser Technique

The objectives of this study is to investigate if pre-treating the skin with an ablative fractional laser that creates small micropores in the skin:

1. Increases the uptake of Methylaminolevulinat (MAL) in the skin.
2. Decreases the required incubation time of MAL when performing photo dynamic therapy.

12 healthy male individuals were included.

On the back, 25 areas measuring 2.8x2.8 cm were marked, plus one control area. We tested 2 variables.

1) Density of laser channels. 2) Methylaminolevulinat (MAL) concentration

1. Laser - Each area was randomized to receiving:

   • no treatment
   • 1% laser channel density
   • 2% laser channel density
   • 5% laser channel density
   • 10% laser channel density
   • 15% laser channel density

2. MAL - Each density was assigned to 4 areas. Within each density category, the four areas were randomized to receive:

   • vehicle
   • 4% MAL
   • 8%MAL
   • 16%MAL

The uptake of MAL was evaluated by fluorescence intensity measured with a florescence camera at t=

• 0min
• 30min
• 1h
• 1.5h 2h 2.5h 3h

Inclusion Criteria:

• Male
• >18 years old
• Fitzpatrick skin type I-III
• No UV exposure thee months prior to study
• Signed study consent

Exclusion Criteria:

• Known allergy to substances in MAL or Unguentum M cream.
• Previous keloid
• Use of topical or systemic photosensitizing drug
• Evaluated to not be able to follow treatment protocol