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| ID | Type | Description | Link |
|---|---|---|---|
| 348525 | Other Identifier | AdventHealth |
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| Name | Class |
|---|---|
| Sanford-Burnham Medical Research Institute | OTHER |
| Pennington Biomedical Research Center | OTHER |
| Boston University | OTHER |
| Tufts University |
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The purpose of this study is to collect data to help understand why some women develop Polycystic Ovary Syndrome (PCOS) associated with decreased lower-body fat.
Epigenetics may represent a new regulator mechanism explaining gluteal vs. abdominal fat differences.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| PCOS group | 12 women with Polycystic Ovary Syndrome (PCOS) as defined by NIH criteria | ||
| Pear shapes | 12 with an android pattern as defined by a waist-to-hip greater than 0.85 | ||
| Apple shapes | 12 will have a gynoid pattern as defined by a waist-to-hip ratio less than 0.78 |
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| Measure | Description | Time Frame |
|---|---|---|
| Difference of angiogenesis capacity in abdominal and gluteal adipose tissue | The angiogenesis capacity of each adipose tissue depot will be determine by an in vitro angiogenesis test. | Day 7 |
| Measure | Description | Time Frame |
|---|---|---|
| Presence of biomarkers in abdominal and gluteal adipose tissue in obese and PCOS women | Measurement will be obtained with Fluorescence-activated cell sorting (FACS). Identify different and similar biomarkers in obese and PCOS women, from abdominal and gluteal adipose tissue samples. Total RNA, micro RNA and DNA will be extracted from each type of cells using FACS-cell sorting analysis. | Day 7 |
| Measure | Description | Time Frame |
|---|---|---|
| The rates of carbohydrate oxidation | Resting Metabolic Rate (RMR) will measure the resting metabolic rate/respiratory quotient (RMR/RQ) and substrate utilization. Energy expenditure and respiratory quotient standardized for temperature, pressure, and moisture will be calculated at one-minute intervals, over 30 minutes. | Day -7 |
Inclusion Criteria:
Supplemental inclusion criteria for PCOS women:
Exclusion Criteria:
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Study Population and screening: Healthy young women with a BMI > 30 will be recruited; 12 will have a gynoid pattern as defined by a waist-to-hip ratio less than 0.78 and 12 with an android pattern as defined by a waist-to-hip greater than 0.85. A third group will include 12 women with PCOS as defined by the NIH criteria. Subjects will be recruited via a dedicated website, physician referrals, direct mail outs, newspaper advertisements, and brochures. Only subjects that will sign informed consent and meet eligibility criteria will be enrolled in this study.
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| Name | Affiliation | Role |
|---|---|---|
| Adeline Divoux, PhD | Translational Research Institute for Metabolism and Diabetes | Principal Investigator |
| Steven R Smith, MD | Translational Research Institute for Metabolism and Diabetes | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Translational Research Institute for Metabolism and Diabetes | Orlando | Florida | 32804 | United States |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 15788499 | Background | Ehrmann DA. Polycystic ovary syndrome. N Engl J Med. 2005 Mar 24;352(12):1223-36. doi: 10.1056/NEJMra041536. No abstract available. | |
| 10956251 | Background | Weber RV, Buckley MC, Fried SK, Kral JG. Subcutaneous lipectomy causes a metabolic syndrome in hamsters. Am J Physiol Regul Integr Comp Physiol. 2000 Sep;279(3):R936-43. doi: 10.1152/ajpregu.2000.279.3.R936. |
| Label | URL |
|---|---|
| Home site of the Translational Research Institute for Metabolism and Diabetes | View source |
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| ID | Term |
|---|---|
| D011085 | Polycystic Ovary Syndrome |
| D008659 | Metabolic Diseases |
| D050177 | Overweight |
| D009765 | Obesity |
| ID | Term |
|---|---|
| D010048 | Ovarian Cysts |
| D003560 | Cysts |
| D009369 | Neoplasms |
| D010049 | Ovarian Diseases |
| D000291 |
Not provided
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| OTHER |
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Blood Urine Adipose tissue
| Difference in oxygen content of abdominal and gluteal adipose tissue | Adipose tissue oxygen content will be measured using the Licox CMP microprocessor. | Day 6 |
| The rates of lipid oxidation |
Resting Metabolic Rate (RMR) will measure the resting metabolic rate/respiratory quotient (RMR/RQ) and substrate utilization. Energy expenditure and respiratory quotient standardized for temperature, pressure, and moisture will be calculated at one-minute intervals, over 30 minutes. |
| Day -7 |
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| 30477572 | Derived | Divoux A, Sandor K, Bojcsuk D, Talukder A, Li X, Balint BL, Osborne TF, Smith SR. Differential open chromatin profile and transcriptomic signature define depot-specific human subcutaneous preadipocytes: primary outcomes. Clin Epigenetics. 2018 Nov 26;10(1):148. doi: 10.1186/s13148-018-0582-0. |
| 28649807 | Derived | Divoux A, Xie H, Li JL, Karastergiou K, Perera RJ, Chang RJ, Fried SK, Smith SR. MicroRNA-196 Regulates HOX Gene Expression in Human Gluteal Adipose Tissue. Obesity (Silver Spring). 2017 Aug;25(8):1375-1383. doi: 10.1002/oby.21896. Epub 2017 Jun 25. |
| Adnexal Diseases |
| D005831 | Genital Diseases, Female |
| D052776 | Female Urogenital Diseases |
| D005261 | Female Urogenital Diseases and Pregnancy Complications |
| D000091642 | Urogenital Diseases |
| D000091662 | Genital Diseases |
| D006058 | Gonadal Disorders |
| D004700 | Endocrine System Diseases |
| D009750 | Nutritional and Metabolic Diseases |
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D001835 | Body Weight |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |