Main objective: To determine the optimal molar doses of the biospecific antibody TF2 and 68 Ga-IMP-288 and the optimal time for pretargeting for immuno-PET in patients with breast carcinoma.

Secondary objectives: To study the sensitivity of the immuno-PET, compare its performance to standard imaging methods, evaluate the safety of 150 MBq of 68 Ga-IMP-288; study the development of immunization against TF2 or complex TF2-IMP-288;

• 4 or 5 cohorts of 3 patients receiving different doses of TF2 IMP-288 with different interval time. A last cohort (4 or 5 ): maximum of 21 additional patients with the optimal schedule.

Cohort I: TF2 120 nmol / 6 nmol IMP-288 / 24 hours

Cohort II: based on the results of the cohort I :

1. Good signal of the tumor but high background: increased interval time, 120 nmol TF2 / 6 nmol of IMP-288 / 30 hours

2. Low signal of the tumor: reduction of the interval time, 120 nmol TF2 / 6 nmol IMP-288 / 18 hours

3. Good signal of the tumor and good background signal : dose reduction, 60 nmol TF2 / 3nmol IMP- 288 / 24 hours

   Cohort III: based on results of cohort II:

   - Good signal of the tumor : dose reduction, 120 nmol TF2 / 3 nmol IMP-288 / 30 h

   Cohort IV : based on results of cohort III Cohort V : Based on results of cohort IV

   • A last Cohort (VI) : 19 patients with the optimal schedule of injection : 120 nmol TF2 / 3 nmol IMP-288 / 30 h or 120 nmol TF2 / 6 nmol IMP-288 / 30 h

   • In the four weeks prior to the immuno-PET:

   - Clinical examination,

   - CEA and CA15-3,

   - thoraco abdominal pelvic scan, bone scan, FDG-PET,

   - immunohistochemistry ACE on the tumor if possible,

   - Anti-Antibodies if the patient has already received MAb,

   - pregnancy test within 2 days prior to immuno-PET,

   - (creatinine > 2.5 normal) D0: Injection of TF2 D1 to D4: injection of 68 Ga-IMP-288 (depending of the cohort) D0 to D4 : pharmacokinetics, imaging

   Evaluation at 1 month of Immuno-PET:

   • Assessment of the clinical oncologist and

   - histological biopsy and / or surgery performed according to the results of imaging and assessment of the potential clinical impact

   Evaluation at 3 and 6 months of immuno-PET:

   based on the results of immuno-PET, evaluation and therapeutic decision of the oncologist,

   - Imaging (ultrasound, bone scintigraphy, CT or PET FDG),

   - markers

   • Anti-Antibody Search
   • For patient with a cancer treatment a new immuno-PET can be proposed

Inclusion Criteria:

• Breast carcinoma, HER2 + (Dako) and HER2 + (fish) metastatic at least after treatment with current consensus

• ≥ 18 years

• Negative pregnancy test for women of childbearing age. Women of childbearing age should take effective contraception continuously for 3 months.

• Karnofsky ≥ 70 or ECOG 0-1

  •• ACE of the tumor by immunohistochemistry or positive plasma CEA ≥ 10 ng / mL

• At least one measurable lesion on CT

• creatinine < 2.5

• Informed consent signed

• Social insurance

Exclusion Criteria:

• Pregnancy or breastfeeding

  • Serious illness or co-morbidity risk assessed
  • History of cancer within 5 years except skin cancer other than melanoma or carcinoma in situ of the cervix
  • Presence of anti-antibodies in patients who have previously received antibodies
  • Known hypersensitivity to antibodies or proteins
  • intellectual disability to sign the informed consent
  • Not controlled diabetes
  • Persons protected by law