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| Name | Class |
|---|---|
| Top Institute Food and Nutrition | OTHER |
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The major research objective is to assess the potential of Proton Magnetic Resonance Spectroscopy to follow Intrahepatic lipid and Intramyocellular lipid dynamics after a single meal. Furthermore we aim to investigate whether the addition of protein to a high fat breakfast results in a reduced postprandial accumulation of lipids when compared to a high fat breakfast alone.
Fat accumulation in non-adipose tissue, such as liver and muscle, is associated with reduced insulin sensitivity and cardiovascular disease. Little is known about the dietary determinants and the time course of this ectopic fat accumulation. Therefore the general aim of this project is to develop and validate methodology to follow postprandial hepatic and muscular lipid retention. Proton Magnetic Resonance Spectroscopy (1H-MRS) is a well-established tool for noninvasive quantification of intrahepatic lipids (IHL) and intramyocellular lipids (IMCL). Previous studies have suggested a relatively rapid regulation of IHL and IMCL levels, indicated by changes in these total lipid pools after high fat diets of 3 or 4 days. Although IHL and IMCL levels seem to change rapidly, data on IHL and IMCL levels after a single meal are lacking.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| High fat breakfast lean subjects | Other | Subjects will consume a high fat breakfast, containing of sausage rolls. Energy content of the breakfast will be set to 50 % of the recommended daily calorie intake (BMR times 1.3). |
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| High fat breakfast,obese subjects | Other | Subjects will consume a high fat breakfast, containing of sausage rolls. Energy content of the breakfast will be set to 50 % of the recommended daily calorie intake (BMR times 1.3). |
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| HF breakfast with protein,lean subjects | Other | For the HFP breakfast, the subjects will consume 'Protifar' (Nutricia, Cuijk, The Netherlands) in addition to the sausage rolls. The amount of added proteins will be calculated to assure that the total energy content from proteins in the breakfast equals 20 %, |
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| HF breakfast with protein,obese subjects | Other | For the HFP breakfast, the subjects will consume 'Protifar' (Nutricia, Cuijk, The Netherlands) in addition to the sausage rolls. The amount of added proteins will be calculated to assure that the total energy content from proteins in the breakfast equals 20 %, |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| High fat breakfast | Dietary Supplement | The HF breakfast will consist of sausage rolls ("saucijzenbroodjes"), available at the local Albert Heijn (Albert Heijn B.V., Zaandam, The Netherlands). |
| Measure | Description | Time Frame |
|---|---|---|
| Difference between pre- and postprandial IHL and IMCL | The primary research objective is to assess the potential of 1H-MRS to follow IHL and IMCL dynamics after a single meal in lean and obese subjects. | 3 and 5 hours after high fat breakfast |
| Measure | Description | Time Frame |
|---|---|---|
| Difference between postprandial IHL and IMCL in high fat and high fat with extra protein breakfast | We aim to investigate whether the addition of protein to a high fat breakfast (HFP) results in a reduced postprandial accumulation of lipids when compared to a high fat breakfast (HF) alone. | 3 and 5 hours after high fat breakfast |
| Measure | Description | Time Frame |
|---|---|---|
| Fat and fat free mass of subjects | Body composition of the subjects is measured with underwaterweighing method | One week before the first MR measurements |
| Blood plasma insulin levels | Ten minutes prior to high fat breakfast and 0.5, 1,2,3.5,4.5,5.5 hours after the high fat breakfast |
Inclusion Criteria:
Exclusion Criteria:
Any medical condition requiring treatment and/or medication use
Alcohol consumption of more than 20 g per day (± 2 units)
Smoking
Unstable body weight (weight gain or loss > 3 kg in the past three months)
Participation in another biomedical study within 1 month prior to the screening visit
Contraindications for MRI scan:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Maastricht University Medical Center + | Maastricht | Netherlands |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 25527751 | Derived | Lindeboom L, Nabuurs CI, Hesselink MK, Wildberger JE, Schrauwen P, Schrauwen-Hinderling VB. Proton magnetic resonance spectroscopy reveals increased hepatic lipid content after a single high-fat meal with no additional modulation by added protein. Am J Clin Nutr. 2015 Jan;101(1):65-71. doi: 10.3945/ajcn.114.094730. Epub 2014 Nov 5. |
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| ID | Term |
|---|---|
| D011506 | Proteins |
| ID | Term |
|---|---|
| D000602 | Amino Acids, Peptides, and Proteins |
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| HF breakfast with protein | Dietary Supplement | For the HFP breakfast, the subjects will consume 'Protifar' (Nutricia, Cuijk, The Netherlands) in addition to the sausage rolls. |
|
| Blood plasma glucose levels | Ten minutes prior to high fat breakfast and 0.5, 1,2,3.5,4.5,5.5 hours after the high fat breakfast |
| Blood plasma free fatty acid level | Ten minutes prior to high fat breakfast and 0.5, 1,2,3.5,4.5,5.5 hours after the high fat breakfast |
| Blood plasma triglyceride level | Ten minutes prior to high fat breakfast and 0.5, 1,2,3.5,4.5,5.5 hours after the high fat breakfast |