Electroporation will increase the efficiency of DNA priming in terms of immune responses and will lead to a dose sparing DNA vaccine regimen. Furthermore increased DNA vaccine concentration will reduce the number of shots necessary to deliver the full dose and induce comparable immune responses as with lower DNA vaccine concentrations.

Inclusion Criteria:

• Willing to undergo counselling and HIV testing.
• Have a negative antigen/antibody ELISA for HIV infection.
• Able to give informed consent.
• Basic abilities to read and write.
• Satisfactory completion of an assessment of understanding prior to enrolment defined as 90% correct answers after three opportunities to take test.
• Resident of the region where the study is taking place.
• At low risk of HIV infection.
• Verbal assurances for adequate birth control measures.
• Healthy as evidenced by clinical and laboratory measures

Exclusion Criteria:

• At risk of HIV infection.
• Active tuberculosis.
• A history of immunodeficiency, ongoing medical and/or psychiatric condition and/or chronic illness requiring continuous or frequent medical intervention.
• Autoimmune disease.
• Hives and severe eczema.
• Substance abuse problems.
• History of grand-mal epilepsy.
• Received blood or blood products or immunoglobulins in the past 3 months.
• Receiving immunosuppressive therapy such as systemic corticosteroids or cancer chemotherapy.
• Use of experimental therapeutic agents within 30 days of study entry.
• History of cardiac disease