The purpose of this study is to investigate the efficacy and safety of autologous transplantation of Adipose Tissue derived Mesenchymal stem cells (MSCs) in patient with critical limb ischemia

Inclusion Criteria:

• At least 6 months since the onset CLI(Chronic ASO or Buerger disease)
• Patients with luminal stenosis > 50% by leg angiography
• Age is between 20 and 80
• Patients whose Rutherford's class is II-4, III-5 or III-6 (Patients with rest pain or ischemic ulcer/necrosis)
• Patients for whom angioplasty and bypass surgery are not indicated because of anatomical or procedural reasons or frequent reocclusion/restenosis following traditional revascularization
• Patients who can give informed consent themselves in writing

Exclusion Criteria:

• Previous or current history of neoplasm or comorbidity that could impact the patient's survival
• Detection of proximal source of emboli including atrial fibrillation
• Primary hematologic disease, including hypercoagulable states
• Detection of proliferative retinopathy
• Entrapment syndrome
• Alcohol abuse, cocaine amphetamine etc.
• Detection of osteomyelitis
• Uncontrolled DM