Treatment Resistance Related With Gene Expression Profile of Diffuse Large B-cell Lymphoma

Retrospective Analysis of Diffuse Large B-cell Lymphoma: Identification of Factors Associated With Treatment Resistance Via Gene Expression Profile

The investigators perform a retrospective microarray gene expression profiling study of FFPE from a cohort of DLBCL patients with whole genome cDNA mediated Annealing Selection and Ligation (WG-DASL) assay. The investigators also study the pattern of microRNA from patients with diffuse large B-cell lymphoma. The results of gene expression profiles and microRNA is correlated with clinical outcomes of diffuse large B-cell lymphoma.

1. Whole genome cDNA mediated Annealing Selection and Ligation (WG-DASL) assay

   • Diffuse large B-cell lymphoma patients
   • Paraffin-embedded tissue
   • End points: Progression-free survival, overall survival
   • Statistics: Leave one-out method

2. Nano string assay for microRNA

   • Diffuse large B-cell lymphoma patients
   • Paraffin-embedded tissue
   • End points: Progression-free survival, overall survival
   • Statistics: Leave one-out method

Inclusion Criteria:

• Patients diagnosed with diffuse large B-cell lymphoma
• Patients with tissue blocks available for study
• Patients with adequate medical records

Exclusion Criteria:

• Patients diagnosed with other subtypes of lymphoma