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PI moved from Hadassah
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Presently spermatozoa for ICSI are selected based on morphology and motion, without determining if acrosome reaction (AR) had occurred during sperm preparation or not. Although not critical if the sperm is injected into the ooplasm, the occurrence of AR might might be an indicator of better spermatozoon quality and implantation potential, especially in severe OTA cases. It is impossible with conventional plain optic microscopy used in IVF units to determine in vivo (without fixation) if AR has occurred, and as a result spermatozoa are injected randomly. It is readily possible to distinguish AR+ from AR- spermatozoa using polarized light microscopy due to different bifriengance.
In this study the investigators seek to determine, by a prospective RCT, if selecting only acrosome reacted spermatozoa, in severe OTA cases, will improve the outcome of ICSI and the entire IVF process.
A prospective RCT to determine the effect of polscope based spermatozoa selection for ICSI.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Spermatozoa election using a polscope | Experimental | AR+ spermatozoa are selected for ICSI using a polscope |
|
| Control | Active Comparator | Spermatozoa for ICSI are selected based on morphology and motion under conventional light microscopy |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Sperm selection by conventional / polarized light microscopy | Procedure | Selecting spermatozoa for ICSI using conventional and polarized light microscopy |
|
| Measure | Description | Time Frame |
|---|---|---|
| fertilization rate | Determine the impact of AR+ spermatozoa selection of fertilization and in vitro embryonic development. | 24-72 h |
| Measure | Description | Time Frame |
|---|---|---|
| Clinical pregnancy | The occurrence of clinical pregnancies in both arms. | up to 6 weeks |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Yoel Shufaro, MD PhD | HMO | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Hadassah University Hospital | Jerusalem | 91120 | Israel |
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| ID | Term |
|---|---|
| D007246 | Infertility |
| ID | Term |
|---|---|
| D000091662 | Genital Diseases |
| D000091642 | Urogenital Diseases |
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| ID | Term |
|---|---|
| D008859 | Microscopy, Polarization |
| D020554 | Sperm Injections, Intracytoplasmic |
| ID | Term |
|---|---|
| D008853 | Microscopy |
| D003952 | Diagnostic Imaging |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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|
| D008919 |
| Investigative Techniques |
| D005307 | Fertilization in Vitro |
| D027724 | Reproductive Techniques, Assisted |
| D012099 | Reproductive Techniques |
| D013812 | Therapeutics |