Studying Genes in Samples From Younger Patients With Ovarian or Testicular Sex Cord Stromal Tumors

DICER1 Mutations and miRNA in Ovarian and Testicular Sex Cord Stromal Tumors of Childhood

RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors identify biomarkers related to cancer.

PURPOSE: This research trial is studying genes in tumor samples from younger patients with ovarian or testicular sex cord stromal tumors.

OBJECTIVES:

• To assess the role of DICER1 mutations and miRNA pathways in the etiology and progression of ovarian or testicular sex cord stromal tumors.

OUTLINE: Archived tumor tissue samples are analyzed for D1CER1 mutation and mRNA expression by reverse-transcriptase polymerase chain reaction (RT-PCR) and laser micro dissector. Results are then correlated with medical data, including age at diagnosis, diagnosis, pathology report, presenting symptoms, family history, treatment, outcome, and length of follow-up, when available.

DISEASE CHARACTERISTICS:

• Fixed and frozen tissue samples from the ATBR01 B1 tissue bank and from the International Pleuropulmonary Blastoma Registry, Children's Hospital of Boston, and Massachusetts General Hospital

• Tissue samples from patients diagnosed with:

  • Ovarian

    • Sertoli-Leydig cell tumor
    • Juvenile granulosa cell tumor
    • Adult granulosa cell tumor
    • Gynandroblastoma
    • Sex cord stromal tumor with annular tubules
    • Sex cord stromal tumor, undifferentiated
    • Sertoli tumor
    • Leydig tumor

  • Testicular:

    • Granulosa cell tumor
    • Sertoli tumor
    • Leydig tumor
    • Sertoli-Leydig
    • Undifferentiated stromal tumor

PATIENT CHARACTERISTICS:

• Not specified

PRIOR CONCURRENT THERAPY:

• Not specified