Identification of Non Invasive Biomarkers of Immune Endothelial Injury and Repair Associated With Cardiac Allograft Vasculopathy
Identification of Non Invasive Biomarkers of Immune Endothelial Injury and Repair Associated With Cardiac Allograft Vasculopathy
Heart transplantation is the best option for patients with end-stage heart failure. Cardiac allograft vasculopathy (CAV) is the leading cause of death following cardiac transplantation and is not managed by current therapies. Its pathogenesis traduces in an accelerated form of coronary artery disease (CAD) with similarities to atherosclerosis but also particular features of endothelial dysfunction associated to the alloimmune conflict and humoral responses toward the graft. Intravascular ultrasound (IVUS) is the validated invasive method for late CAV diagnosis, but occurs lesions are established. Identification of reliable non-invasive early endothelial injury biomarkers that reflect mechanisms of cardiac damage thus remain a major challenge to optimize therapeutic management of post transplant morbidity. Endothelial dysfunction is a central feature of both CAV and CAD and results from a desquilibrium in the balance of endothelial lesion and repair that is partly controlled by recipient immune system. Through their expression of receptors sensing antibodies (FcR CD16) and endothelial stress-induced signals (CX3CR1 fractalkine receptor and NKG2D MICA receptors), Natural Killer (NK) cells represent effector cells with unique potential to generate both humoral and innate immune injury of graft endothelium.
Inclusion Criteria:
Subject > 18 years at the time of the inclusion,
Subject having benefited from a heart transplant more than 11 months ago in the service of cardiac surgery concerned whatever is the treatment to immunosuppresseur current
Subject benefiting from a coronarography within the framework of their surveillance comment-Clerk's Office beyond 12 months
Subject having given their consent
Affiliated to the Social Security
* HTC with Cardiac allograft vasculopathy:
Subject with coronaropathies diagnosed by the coronarography
* TC without Cardiac allograft vasculopathy:
Subject without coronaropathies diagnosed by the coronarography
* untransplanted
Untreated Subject by immunosuppresseurs
Subject without antécédaent of transfusion
Subject without history of transplantations
Subject with coronaropathies diagnosed by a coronarography
Exclusion Criteria: