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The objective of the study is to evaluate the nutritional and physiological properties of two canola proteins focusing on the bioavailability in humans.
The physiological effects of a single administration of two different industrially manufactured canola proteins (hydrolyzate, isolate) were investigated compared to soy protein.
Twenty-eight healthy men (ø 25 years) completed a double-blind, placebo-controlled, crossover study. The subjects were randomly divided into two groups (group A: canola protein isolate and soy protein isolate and group B: canola protein hydrolyzate and soy protein isolate). After a three-day run-in period, on the intervention day, the subjects consumed a protein drink containing 30.0 g protein powder added to a final volume of 500 mL tomato juice. Half of the subjects in each group consumed a protein drink containing 30.0 g canola protein, the other half received the soy protein. After taking of fasting blood samples in the morning of the intervention day, blood samples were drawn at regular intervals for a period of eight hours. At the end of the run-in period and on the intervention day, a 24-hour urine collection took place.
Within a second experiment (after 4 weeks), the protein source was crossed within the four subgroups for the second intervention.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Canola protein | Experimental | Canola protein (Brassica juncea; incorporated in a drink) |
|
| Reference protein | Active Comparator | Reference Protein (soy protein isolate; incorporated in a drink) |
|
| Wash out | No Intervention | Wash out (four weeks without any intervention between interventional periods) | |
| Run-in | No Intervention | Run-in period (three days before intervention, defined diet) |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Protein drink - protein isolate | Dietary Supplement | The subjects consumed 500 mL of a protein drink (tomato juice) containing 30 g protein (canola protein isolate or reference protein) after overnight fasting. After run-in period, each volunteer have to pass both protein-intervention days in different order with a four-week wash out period in between. |
| Measure | Description | Time Frame |
|---|---|---|
| Plasma amino acids | Free proteinogenic amino acids | At Day 1 (Intervention day); After 0, 30, 60, 90, 120, 180, 240, 300, 360, 420 and 480 minutes |
| Measure | Description | Time Frame |
|---|---|---|
| Body composition (body status) | Bioelectrical impedance analysis, body weight | At day 1 |
| Nitrogen balance | Nitrogen balance = nitrogen intake - nitrogen output (urine nitrogen + fecal nitrogen + skin nitrogen); Nitrogen intake was calculated from food uptake; Urine nitrogen contains urea, uric acid, creatinine and total protein; Fecal nitrogen was calculated as the tenth part of the nitrogen intake; Skin nitrogen was calculated as 5.00 mg per kg of body weight |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Gerhard Jahreis, Prof. Dr. | Friedrich Schiller University Jena, Institute of Nutrition, Department of Nutritional Physiology | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Friedrich Schiller University Jena, Department of Nutritional Physiology | Jena | Thuringia | 07743 | Germany |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 23260747 | Derived | Fleddermann M, Fechner A, Rossler A, Bahr M, Pastor A, Liebert F, Jahreis G. Nutritional evaluation of rapeseed protein compared to soy protein for quality, plasma amino acids, and nitrogen balance--a randomized cross-over intervention study in humans. Clin Nutr. 2013 Aug;32(4):519-26. doi: 10.1016/j.clnu.2012.11.005. Epub 2012 Nov 14. |
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| ID | Term |
|---|---|
| D018761 | Multiple Endocrine Neoplasia Type 1 |
| ID | Term |
|---|---|
| D009377 | Multiple Endocrine Neoplasia |
| D004701 | Endocrine Gland Neoplasms |
| D009371 | Neoplasms by Site |
| D009369 | Neoplasms |
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| ID | Term |
|---|---|
| C110747 | cyclopropapyrroloindole |
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|
|
| Protein drink - protein hydrolyzate | Dietary Supplement | The subjects consumed 500 mL of a protein drink (tomato juice) containing 30 g protein (canola protein hydrolyzate or reference protein) after overnight fasting. After run-in period, each volunteer have to pass both protein-intervention days in different order with a four-week wash out period in between. |
|
|
| After day 0 and 1 |
| D009378 |
| Neoplasms, Multiple Primary |
| D009386 | Neoplastic Syndromes, Hereditary |
| D030342 | Genetic Diseases, Inborn |
| D009358 | Congenital, Hereditary, and Neonatal Diseases and Abnormalities |
| D004700 | Endocrine System Diseases |