Optimised Fluoride Prevention by Double Rinse With Fluoride and Calcium

A Double Blind, Randomized, Cross Over Designed Trial of Fluoride Prevention by Double Rinse With Calcium and Fluoride

Aim: to determine the optimal Ca2+ concentration with 905 ppm F as NaF. Study design: Experimental study in 10 volunteers. Single blind mouth rinse with calcium lactate solution (150; 75; 0 mM Ca-lactate) is immediately followed by a standard fluoride rinse.

Procedure: Rinses are performed in the evening. The rinse combinations are given in a random order, and the subjects are unaware of the sequence. At least 3 days separates the use of each rinse. Dosage: 20 mL and 1 minute rinse with each solution.

Saliva samples: Twelve hours after rinsing, unstimulated saliva samples are collected by expectoration.

Analysis: The fluoride concentration in saliva samples are analysed Statistics and data handling: Fluoride in saliva 12 hours after rinsing are examined by one-way ANOVA, repeated measures design.

Inclusion Criteria:

• 18 years or older
• 10 natural teeth or more
• willing to refrain from use of fluoride containing products
• signed informed consent

Exclusion Criteria:

• less than 10 natural teeth
• reduced cognitive skills
• does not speak and/or understand Swedish
• ongoing oral or systemic infections
• pregnancy
• breast feeding