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Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animals. The investigators aim at generating haploid stem cells from human germ cells that are a useful tool in genetic research and screening.
Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animal models. haploid cells are easier for genetic manipulations, such as knock-in and knock-out of genes, and therefore should make a better experimental model. The investigators aim at generating haploid stem cells from human haploid germ cells.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Sperm sample from healthy volunteers |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| generation of stem cells | Other | Collected germ cells will be cultured in medium. The germ cell cultures will be infected with reprograming factors. Rare colonies with characteristic stem cell morphologies will be expanded. |
| Measure | Description | Time Frame |
|---|---|---|
| Patients donated samples | No samples collected to date | June 2011-November 2013 |
| Measure | Description | Time Frame |
|---|---|---|
| No secomdary outcome | June 2011 - November 2013 |
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Inclusion Criteria:
Exclusion Criteria:
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Male volunteers
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| Name | Affiliation | Role |
|---|---|---|
| Itay Maza, M.D | Rambam Health Care Campus | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Rambam medical center | Not yet recruiting | Haifa | 30196 | Israel |
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Germ cells and Stem cells
|
| Weizmann institute of science | Recruiting | Rehovot | 76100 | Israel |
|