A study to evaluate the safety, tolerability, pharmacokinetics and pharmacodynamics of multiple ascending dose of ASP0456 in non-elderly healthy subjects. Two dosing condition, pre-prandial and postprandial administrations, is compared.

Inclusion Criteria:

• Healthy, as judged by the investigator/subinvestigator based on the results of physical examination and all tests obtained

• Body weight (at screening);

  • female ≥40.0 kg,<70.0 kg
  • male ≥50.0 kg,<80.0 kg

• BMI(at screening): ≥17.6,<26.4

Exclusion Criteria:

• Received any investigational drugs in other clinical or post-marketing studies within 120 days before the study
• Donated 400 mL of whole blood within 90 days, 200 mL of whole blood within 30 days, or blood components within 14 days before the study
• Received medication within 10 days before dose is administered or is scheduled to receive medication
• History of drug allergies
• Upper (e.g. nausea, vomiting, stomachache) and lower (e.g. abdominal pain) gastrointestinal disease within 7 days before the study
• Concurrent or previous hepatic disease
• Concurrent or previous heart disease
• Concurrent or previous renal disease