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The purpose of this study is to determine if the phase of the menstrual cycle (opposed versus unopposed estrogen phase) affects eating behavior, including types of foods preferred, wanting/desire for specific foods, and perception of hunger.
This is an observational study to investigate the effect of endogenous estrogen (E2) and progesterone (P4) levels on eating behavior and energy-yielding nutrient metabolism. Eating behavior will be assessed during a complete menstrual cycle during the first month of the study. Participants will track the follicular and luteal phases of their menstrual cycle using urinary leuteinizing hormone (LH) kits, they will record information about their food intake, eating behaviors, and appetite in their home environments, and they will complete two laboratory test days: one test day scheduled during their follicular phase, and one test day scheduled during their luteal phase.
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| Measure | Description | Time Frame |
|---|---|---|
| Eating behavior | Eating behavior, including types of foods preferred, wanting/desire for specific foods, and perception of hunger will be measured using four questionnaires; Eating inventory: to assess dietary restraint, Stanford Brief Physical Activity Questionnaire, Food craving questionnaire, Eating behavior and attitudes questionnaire | At follicular (day 11) and luteal (day 26) phase of menstrual cycle |
| Measure | Description | Time Frame |
|---|---|---|
| Insulin sensitivity | To determine if the phase of the menstrual cycle affects insulin sensitivity, we will measure plasma insulin, glucose and non-esterified fatty acids (NEFA). | At follicular (day 11) and luteal (day 26) phase of menstrual cycle |
| Serum lipid profile changes |
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Inclusion Criteria:
Exclusion Criteria:
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Healthy, premenopausal women.
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| Name | Affiliation | Role |
|---|---|---|
| Nancy Keim, PhD | USDA, ARS, Western Human Nutrition Research Center | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| USDA, ARS, Western Human Nutrition Research Center | Davis | California | 95616 | United States |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 30031087 | Derived | Krishnan S, Agrawal K, Tryon RR, Welch LC, Horn WF, Newman JW, Keim NL. Structural equation modeling of food craving across the menstrual cycle using behavioral, neuroendocrine, and metabolic factors. Physiol Behav. 2018 Oct 15;195:28-36. doi: 10.1016/j.physbeh.2018.07.011. Epub 2018 Jul 18. | |
| 27527001 | Derived |
| Label | URL |
|---|---|
| United States Department of Agriculture, ARS, Western Human Nutrition Research Center | View source |
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| ID | Term |
|---|---|
| D005247 | Feeding Behavior |
| D005518 | Food Preferences |
| ID | Term |
|---|---|
| D001522 | Behavior, Animal |
| D001519 | Behavior |
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Serum, plasma and urine samples may be retained for future testing.
Blood samples will be analyzed for lipids and lipoproteins, including apoproteins A1, B, C-II, C-III, and E. |
| At follicular (day 11) and luteal (day 26) phase of menstrual cycle |
| Urinary c-peptide | The level of urinary C-peptide and creatinine will be measured in a 24-hour urine collection. | At follicular (day 11) and luteal (day 26) phase of menstrual cycle |
| Estradiol | Estradiol measured in serum in relation to appetite variables | At follicular (day 11) and luteal (day 26) phase of menstrual cycle |
| progesterone | Progesterone measured in serum in relation to appetite variables | At follicular (day 11) and luteal (day 26) phase of menstrual cycle |
| Dehydroepiandrosterone (DHEA) | Dehydroepiandrosterone measured in serum in relation to appetite variables | At follicular (day 11) and luteal (day 26) phase of menstrual cycle |
| Sex Hormone Binding Globulin (SHBG) | SHBG measured in serum in relation to appetite variables | At follicular (day 11) and luteal (day 26) phase of menstrual cycle |
| Endocannabinoid profile | Endocannabinoid profile measured in serum to assess appetite | At follicular (day 11) and luteal (day 26) phase of menstrual cycle |
| Leptin | Leptin measured in serum to assess appetite | At follicular (day 11) and luteal (day 26) phase of menstrual cycle |
| Krishnan S, Tryon RR, Horn WF, Welch L, Keim NL. Estradiol, SHBG and leptin interplay with food craving and intake across the menstrual cycle. Physiol Behav. 2016 Oct 15;165:304-12. doi: 10.1016/j.physbeh.2016.08.010. Epub 2016 Aug 12. |