The objective of this study is to investigate the potential beneficial effect of increasing protein in the diet in order to decrease hepatic lipid accumulation on a high-fat diet.

The investigators hypothesize that increasing protein in a high-fat diet suppresses lipid accumulation in the liver, and that changes in (hepatic) fat handling underlie this reduced lipid accumulation.

Inclusion Criteria:

• Healthy
• body mass index (BMI) 18-25 kg/ m2;
• stable dietary habits;
• physical activity levels.
• caucasian

Exclusion Criteria:

• Unable or unwilling to comply with study procedures;
• not caucasian
• Unstable body weight (weight gain or loss > 3 kg in the past three months);
• Moderate intense physical activity (exercise) for more than 4 hours/week;
• (Chronic) disease which might influence the study outcomes e.g. diabetes mellitus or any other endocrine disorder, active cardiovascular disease, hepatic disease, renal disease, cancer;
• Family history of diabetes mellitus;
• Use of medication, except incidental use of paracetamol;
• Abuse of drugs;
• Alcohol consumption of more than 14 glasses per week;
• Participation in another biomedical study within 1 months prior to the first screening visit;
• Contraindications to MRI scanning. These contraindications include patients with one of the following conditions:
• Claustrophobia;
• Central nervous system aneurysm clips;
• Implanted neural stimulator;
• Implanted cardiac pacemaker or defibrillator;
• Cochlear implant;
• Ocular foreign body (e.g. metal shavings);
• Insulin pump;
• Metal shrapnel or bullet;
• Or metal containing corpora aliena in the eye of brains.