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The purpose of this study is to establish if cells present in lung tissue during extrinsic allergic alveolitis (EAA) are able to elicit an immune response against specific antigens. The hypothesis is: cells in BAL lavage fluid from patients with EAA will be responsive to specific antigens and this response can be measured using ELISPOT.
Subjects suffering from extrinsic allergic alveolitis caused by pigeons will undergo a broncho alveolar lavage. Cells obtained from this BAL will be used for ELISPOT technique with pigeon specific antigens
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| non-EAA, pigeon | subject with BAL because of diseases not suspected to be EAA | ||
| EAA, pigeon | Bird fanciers with a typical clinical presentation suspected for pigeon induced EAA |
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| Measure | Description | Time Frame |
|---|---|---|
| ELISPOT response in cells obtained from broncho-alveolar lavage | All elispots will be performed the same day, the end point will be available the next day | 2 days |
| Measure | Description | Time Frame |
|---|---|---|
| Elispot response in cells from blood | ELispots will be performed the same day as blood collection, results will be available the next day | 2 days |
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Inclusion Criteria:
Exclusion Criteria:
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Patients suspected to have pigeon induced extrinsic allergic alveolitis will be recruited by advertisment in bird fanciers journals. Controls will be patients at the department of pulmonology and tuberculosis needing examination for other reasons
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| Name | Affiliation | Role |
|---|---|---|
| Aik Bossink, MD PhD | Diakonessenhuis, Utrecht | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Diakonessenhuis Utrecht | Utrecht | 3582 KE | Netherlands |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 19590020 | Background | Jafari C, Thijsen S, Sotgiu G, Goletti D, Dominguez Benitez JA, Losi M, Eberhardt R, Kirsten D, Kalsdorf B, Bossink A, Latorre I, Migliori GB, Strassburg A, Winteroll S, Greinert U, Richeldi L, Ernst M, Lange C; Tuberculosis Network European Trialsgroup. Bronchoalveolar lavage enzyme-linked immunospot for a rapid diagnosis of tuberculosis: a Tuberculosis Network European Trialsgroup study. Am J Respir Crit Care Med. 2009 Oct 1;180(7):666-73. doi: 10.1164/rccm.200904-0557OC. Epub 2009 Jul 9. | |
| 17715165 |
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| ID | Term |
|---|---|
| D000542 | Alveolitis, Extrinsic Allergic |
| ID | Term |
|---|---|
| D017563 | Lung Diseases, Interstitial |
| D008171 | Lung Diseases |
| D012140 | Respiratory Tract Diseases |
| D012130 | Respiratory Hypersensitivity |
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Cells obtained from Broncho alveolar lavage and blood collection
| Background |
| Losi M, Bossink A, Codecasa L, Jafari C, Ernst M, Thijsen S, Cirillo D, Ferrarese M, Greinert U, Fabbri LM, Richeldi L, Lange C; European Tuberculosis Network TBNET. Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy. Eur Respir J. 2007 Dec;30(6):1173-9. doi: 10.1183/09031936.00067307. Epub 2007 Aug 22. |
| 18193383 | Background | Kosters K, Nau R, Bossink A, Greiffendorf I, Jentsch M, Ernst M, Thijsen S, Hinks T, Lalvani A, Lange C. Rapid diagnosis of CNS tuberculosis by a T-cell interferon-gamma release assay on cerebrospinal fluid mononuclear cells. Infection. 2008 Dec;36(6):597-600. doi: 10.1007/s15010-007-7316-0. Epub 2008 Jan 12. |
| D006969 | Hypersensitivity, Immediate |
| D006967 | Hypersensitivity |
| D007154 | Immune System Diseases |