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researchers moved to new institution and did not transfer project
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The purpose of this study is to study impairment of white blood cell function in patients with type II diabetes.
Leucocytes from poorly controlled diabetes exhibit aberrant chemotaxis, increased susceptibility to bacterial infection, leukotriene production, lysosomal enzyme release, proinflammatory cytokine expression and production of reactive oxygen species. Aberrant glucose concentration in diabetics affects functions of peripheral blood system as well as the immune system leading to impaired host defense. Impaired wound healing is a serious complication associated with diabetes. We hypothesized that impairment in leukocyte function results in dysfunctional inflammatory response in diabetic wounds. The proposed studies focus on characterizing mechanisms that will improve our understanding of the dysfunctional inflammatory response resulting in non-healing chronic wounds in diabetics.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Normoglycemic | Healthy subjects that do not have diabetes | ||
| Type II Diabetes (HbA1c <7 or 7%) | Subject that have Type II Diabetes with good glucose control with glycated hemoglobin (HbA1c <7 or 7%) | ||
| Type II Diabetes (HbA1c between 7.1-9) | Subjects with Type II Diabetes with moderate glucose control (HbA1c between 7.1-9) | ||
| Type II Diabetes (HbA1c >9%) | Subjects with Type II Diabetes with poor glucose control (HbA1c >9%) |
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| Measure | Description | Time Frame |
|---|---|---|
| Ex vivo leukocyte function by measuring ROS production | After blood draw monocytes are separated from whole blood and production of oxidants by these cells | immediately after blood draw |
| Measure | Description | Time Frame |
|---|---|---|
| Ex vivo NADPH oxidase gene and protein expression | Gene and protein expressions are measured using Western blot and real time PCR. | After blood draw |
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Inclusion Criteria:
Exclusion Criteria:
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Subjects will be recruited from the population who visit the Ohio State University (OSU) Hospitals and Comprehensive Wound Center (CWC), OSU Wexner Medical Center diabetic clinics and Bariatric clinic.
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| Name | Affiliation | Role |
|---|---|---|
| Roy Sashwati, MS, PhD | Ohio State University Dept of Surgery | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Ohio State University Comprehensive Wound Center | Columbus | Ohio | 43221 | United States |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 11194213 | Result | Shurtz-Swirski R, Sela S, Herskovits AT, Shasha SM, Shapiro G, Nasser L, Kristal B. Involvement of peripheral polymorphonuclear leukocytes in oxidative stress and inflammation in type 2 diabetic patients. Diabetes Care. 2001 Jan;24(1):104-10. doi: 10.2337/diacare.24.1.104. | |
| 8390941 | Result | Lin X, Candlish JK, Thai AC. Superoxide production by neutrophils from diabetics and normal subjects in response to glucose and galactose. Exp Mol Pathol. 1993 Jun;58(3):229-36. doi: 10.1006/exmp.1993.1020. |
| Label | URL |
|---|---|
| OSU Center for Regenerative Medicine and Cell Based Therapies | View source |
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| ID | Term |
|---|---|
| D003924 | Diabetes Mellitus, Type 2 |
| ID | Term |
|---|---|
| D003920 | Diabetes Mellitus |
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
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Blood
| 971792 | Result | Molenaar DM, Palumbo PJ, Wilson WR, Ritts RE Jr. Leukocyte chemotaxis in diabetic patients and their nondiabetic first-degree relatives. Diabetes. 1976;25(2 SUPPL):880-3. |
| 4826528 | Result | Hill HR, Sauls HS, Dettloff JL, Quie PG. Impaired leukotactic responsiveness in patients with juvenile diabetes mellitus. Clin Immunol Immunopathol. 1974 Apr;2(3):395-403. doi: 10.1016/0090-1229(74)90057-9. No abstract available. |
| OSU Comprehensive Wound Center | View source |
| D004700 | Endocrine System Diseases |