Peripheral Blood Stem Cell Transplantation Using the CliniMACS Device

CHP 834 Unrelated and Partially Matched Related Donor Peripheral Stem Cell Transplantation With The CliniMACS Device for T and B Cell Depletion

T cell depletion utilizing the CliniMACS device will allow more precise, specific and controlled graft engineering of peripheral blood stem cells from unrelated and partially matched related donors without an increase in relapse or graft rejection and grade III or IV acute graft versus host disease (GVHD).

Inclusion Criteria:

• All races are eligible
• Malignant diseases: Leukemias and Lymphomas
• Non-malignant diseases: Severe Aplastic Anemia, immunodeficiencies

Exclusion Criteria:

• Lansky or Karnofsky > 70
• Echo > 27% shortening fraction
• renal function:serum creatinine < 1.5 x for normal age
• no active untreated infection
• DLCO > 50% of predicted value
• Hepatic: AST and ALT < 3x upper limit of normal; bilirubin < 2.0.