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| ID | Type | Description | Link |
|---|---|---|---|
| NBIB |
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The study was closed due to the departure of several of the co-investigators.
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Currently there is no technique to produce thin (0.004-0.01 mm) serial sections of large fresh tissue specimens that are suitable for high-resolution in situ protein/gene expression studies without ice artifact or fixation-induced molecular damage. Traditional frozen sectioning preserves protein and nucleic acid structure, but the inherent ice artifact precludes reconstruction of protein and mRNA expression patterns in 3-dimensions. Since the limitations of the existing sectioning techniques result from the fact that they rely on mechanical cutting which in turn require the tissue to be stiff, we suggest a new approach to cut tissue via an electro erosion process that utilizes focus radio frequency (RF).
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Tissue Sectioning via Electro Erosion Process | Other | Focused RF energy can be used to produce consecutive thin sections of fresh tissue for immunohistochemical and nucleic acids analysis by electro-dissociation without thermal damage, ultimately allowing high resolution reconstruction of gene and protein expression patterns of large tissue specimens in 3D. |
Inclusion Criteria:
Exclusion Criteria:
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Discarded human tissue obtained immediately following surgical resection
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| Name | Affiliation | Role |
|---|---|---|
| Gal Shafirstein, Ph.D. | UAMS, ACH, Central Arkansas Veterans Healthcare System | Principal Investigator |
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