To evaluate two paradigms for simultaneous assessment of hepatic and intestinal CYP3A activity.

Inclusion Criteria:

• Male or non-pregnant female, 18-40 yr. old
• Good general health with no remarkable medical conditions such as liver, kidney, heart, or lung failure
• BMI between 20-33
• Provide informed consent

Exclusion Criteria:

• Known history of liver or kidney disease
• Use of prescription or non prescription medications, herbals or foods known to be metabolized by or affect CYP3A (including oral birth control medications)
• Females who are pregnant or nursing
• Known history of drug or alcohol addiction (prior or present addiction or treatment for addiction)
• Direct physical access to and routine handling of addicting drugs in the regular course of duty (this is a routine exclusion from studies of drugs with addiction potential).
• History of bradycardia
• Respiratory rate <10
• History of significant pulmonary disease
• History of pre-existing medical condition predisposing to respiratory depression
• Systolic blood pressure <100 mgHg and diastolic blood pressure <70mmHg

1 year