The purpose of this study is to gain knowledge about how participants behave and respond on their own after completing a 2-year intervention of calorie restriction.

You will not be assigned to any particular group during the Follow-Up Study. In the Follow-Up study there is no diet intervention. You will not be asked to follow a calorie-restricted diet or any other diet.

Month 3 and Month 6 (Outpatient Visits)following procedures performed:

• Blood pressure, heart rate, temperature, Weight & Waist Measurement
• Medication and medical history
• Blood draw (approximately ½ cup)
• DEXA and QuickScan
• Psychological assessment questionnaires reviewed for completeness
• Body Acceptability Morph Test (BAM)
• Review of 7-day food diary

Month 12 and Month 24 (Outpatient Visits) procedures performed:

• Collection of 4 urine samples
• Dosing with doubly labeled water (DLW)
• DEXA and QuickScan
• Eat breakfast and lunch
• Receive 7-day food diary
• Psychological assessment questionnaires
• Body Acceptability Morph Test (BAM)

Fourteen days after Month 12 and Month 24 (Overnight Inpatient Visits) following procedures performed:

• Collection of 2 urine samples
• Blood pressure, heart rate, temperature, Weight & Waist Measurement
• Blood draw (approximately ½ cup)
• Medication and medical history
• Review of 7-day food diary
• Metabolic Chamber
• Resting Metabolic Rate (RMR)

Inclusion Criteria:

• You may qualify to participate in this study only if you completed the CALERIE II study at Pennington Biomedical Research Center.

Exclusion Criteria:

• If you were not a participant in CALERIE II at Pennington Biomedical Research Center, you are ineligible.

Participants that completeded the CALERIE II study at Pennington Biomedical Research Center.