In the present study, the effects of 3 weeks of unloading on muscle mass and muscle fiber characteristics will be determined. In addition, the effects of 6 weeks of recovery will be assessed.

Inclusion Criteria:

• Healthy
• BMI < 30 kg/m2

Exclusion Criteria:

• Use of medication that may affect haemostasis
• Subjects with (recent) musculoskeletal/orthopaedic disorders known to affect the outcome of the study or that compromise their ability to walk with crutches
• Subjects with metal implants in their lower limbs
• Subjects with known cardiovascular and/or haemostasis disorders
• Subjects with known severe hypertension