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| ID | Type | Description | Link |
|---|---|---|---|
| CDR0000466578 | Registry Identifier | PDQ (Physician Data Query) |
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| Name | Class |
|---|---|
| National Cancer Institute (NCI) | NIH |
RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors predict how well patients respond to treatment.
PURPOSE: This laboratory study is looking at biomarkers that may predict response to tamoxifen and letrozole in postmenopausal women with primary breast cancer treated on clinical trial CAN-NCIC-MA17.
OBJECTIVES:
OUTLINE: This is a controlled study.
Formalin-fixed, paraffin-embedded breast tumor tissue samples are analyzed for MGH 2-gene and GHI 21-gene expression signatures using real-time quantitative polymerase chain reaction. Immunohistochemistry and immunofluorescence are used for analysis of estrogen receptor, progesterone receptor, HER-1 and -2, aromatase, GATA-3, NAT-1, and cyclooxygenase-2. Microarray hybridization is used to identify novel gene expression signatures.
PROJECTED ACCRUAL: A total of 957 specimens will be accrued for this study.
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| microarray analysis | Genetic | |||
| polymerase chain reaction | Genetic | |||
| protein expression analysis | Genetic | |||
| diagnostic laboratory biomarker analysis | Other | |||
| fluorescent antibody technique | Other | |||
| immunohistochemistry staining method | Other | |||
| immunologic technique | Other |
| Measure | Description | Time Frame |
|---|---|---|
| Prognostic utility of the MGH 2-gene and the GHI 21-gene expression signatures | ||
| Ability of the MGH 2-gene and the GHI 21-gene expression signatures to predict responsiveness to letrozole | ||
| Prognostic utility of quantitative immunofluorescence vs standard immunohistochemistry of estrogen receptor, progesterone receptor, HER-2, tumor aromatase, cyclooxygenase-2, GATA-3, and NAT-1 | ||
| Ability of quantitative immunofluorescence and standard immunohistochemistry of these proteins to predict responsiveness to letrozole | ||
| Novel gene expression profiles that may predict outcome and responsiveness to letrozole |
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DISEASE CHARACTERISTICS:
Histologically or cytologically confirmed primary invasive breast carcinoma resected at time of original diagnosis
Treated on clinical trial CAN-NCIC-MA17
Hormone receptor status:
PATIENT CHARACTERISTICS:
PRIOR CONCURRENT THERAPY:
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| Name | Affiliation | Role |
|---|---|---|
| Paul E. Goss, MD, PhD | Massachusetts General Hospital | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Massachusetts General Hospital | Recruiting | Boston | Massachusetts | 02114 | United States |
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| ID | Term |
|---|---|
| D001943 | Breast Neoplasms |
| ID | Term |
|---|---|
| D009371 | Neoplasms by Site |
| D009369 | Neoplasms |
| D001941 | Breast Diseases |
| D012871 | Skin Diseases |
| D017437 | Skin and Connective Tissue Diseases |
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| ID | Term |
|---|---|
| D046228 | Microarray Analysis |
| D016133 | Polymerase Chain Reaction |
| D005455 | Fluorescent Antibody Technique |
| D007150 | Immunohistochemistry |
| D007158 | Immunologic Techniques |
| ID | Term |
|---|---|
| D046208 | Microchip Analytical Procedures |
| D008919 | Investigative Techniques |
| D021141 | Nucleic Acid Amplification Techniques |
| D005821 | Genetic Techniques |
| D006651 | Histocytochemistry |
| D003584 | Cytological Techniques |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D006652 | Histological Techniques |
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