The Effect of Amiloride and Spironolactone in Healthy Persons

The Effect of Amiloride and Spironolactone Measured on Cardiovascular and Kidney Related Variables in Healthy Subjects in a Double-blinded, Randomised, Placebo-controlled, Cross-over Study

This study will investigate whether retaining potassium in the body will lead to changes in blood pressure and changes in blood and urine samples.

The purpose is to measure the effect of amiloride and spironolactone on:

1. Renal function (GFR, u-AQP2, u-ENaCβ, u-cAMP, u-PGE2, CH20, FENa, FEK),
2. Pulsbewave velocity, augmentation index and central blood pressure,
3. Vasoactive hormones (PRC, AngII, Aldo, AVP, ANP, BNP and Endot), and
4. Ambulatory blood pressure

Inclusion Criteria:

• Healthy
• Non-smokers

Exclusion Criteria:

• Smoking
• Under medical treatment