quantitative sweat testing

Blood pressure receptors in the neck arteries may be stimulated by applying suction through a collar around the neck.

IV Pharmacological Testing

phenylephrine 12.5 - 400 ug, isoproterenol 0.1 - 0.4 ug or higher until desired effect, nitroprusside 0.1 - 1.6 ug/kg, propranolol 1.1 mg/min, edrophonium maximum of 10 mg, atropine .01 mg/kg, tyramine 250-4000 ug or higher until desired effect

Oral Pharmacological Testing

clonidine 0.1-0.3 mg, yohimbine 5-10 mg, metoclopramide 10 mg, alpha-methyldopa 62.5 mg, placebo

Determination of body composition

Examination of pressure in the eye and eyelid fatiguability. The following eyedrops might be used:

1. 0.5% proparacaine (Alcaine, Allergan, Inc)
2. Fluress (0.4% benoxinate hydrochloride, fluorescein sodium, Akorn, Inc)
3. 0.5%, 1% tropicamide (Mydriacyl, Alcon)
4. Over-the-counter preservative-free artificial tears
5. 0.25%, 2.5% and 10% phenylephrine (Bausch and Lomb)
6. 1% cyclopentolate hydrochloride (Alcon)

Recording of sleep pattern overnight

Subjects will rate the quality and intensity of 2 pain tasks.

Determination of metabolic rate via 24hr stay in whole-room indirect calorimeter

Questionnaires and computer tasks, an EEG and an MRI may be used to assess brain function.

Blood pressure and heart rate may be monitored while exercising on a stationary bicycle.