Changes of Biomarkers in Response to Training and Antioxidant Treatment

Effects of Training and Antioxidant Treatment on Circulating Markers of Oxidative Stress and Skeletal Muscle mRNA Expression

The aim of the study is to compare the effects of an intensive 4 weeks exercise training program with or without additional treatment with antioxidants. 16 volunteers are included into the study and randomized into a treatment (n=8,Vitamine E 400 IU od + vitamine C 1g twice daily) and a non-treatment (n=8, training only) group. Before and after the training program, skeletal muscle biopsies will be taken to measure PGC-1alpha and PPARgamma mRNA expression. In addition before and after the training program individuals will have the following measurements: body weight, waist circumference, body fat content (DEXA scan), euglycemic-hyperinsulinemic clamps, ergospirometer (VO2 max), routine laboratory measurements, serum malondialdehyde concentrations.

Inclusion Criteria:

• Must be able to swallow tablets
• signed informed consent
• no concommitant medication
• male
• age between 25 - 35 years

Exclusion Criteria:

• drug, tobacco or alcohol abuse
• any chronic disease
• any acute or chronic inflammatory disease as determined by a leucocyte count > 7000 Gpt/l, C-reactive protein (CrP) > 5.0 mg/dl or clinical signs of infection
• systolic blood pressure (SBP) > 140mmHg and diastolic blood pressure (DBP) was > 85mmHg
• cardiovascular or peripheral artery disease
• thyroid dysfunction