We are seeking healthy female volunteers to determine if multinutrient supplementation affects visible signs of skin aging as well as blood measurements of aging. We are seeking smokers and non-smokers.

Skin antioxidant levels as well as blood metabolites may change as the human body ages1,2. Although the chronological aging process is unstoppable, the physical signs of aging, particularly characteristics of the skin, may be delayed through a variety of medical interventions such as topical medications or resurfacing lasers. It is not currently known whether skin carotenoid antioxidant levels correlate with skin aging characteristics, although studies with other antioxidants suggest this may be true 3. In addition, while selected blood metabolites correlate with changes in age2, we do not know if this correlates with skin changes. Our current study focuses on whether differences in skin carotenoid levels and blood metabolite levels correlate with skin aging characteristics in age-matched subjects. Furthermore, we will explore whether three months consumption with a commercial multi-nutrient formulation (LifePak Nano) affects metabolic markers of aging, skin aging characteristics including skin elasticity and transepidermal water loss. We will also examine whether smokers respond differently from nonsmokers in the above parameters after supplementation.

Inclusion Criteria:- Fitzpatrick Skin type I or II

• Nonpregnant Females, age 18-30 or 50-70
• Body Mass Index Normal or Overweight
• If age 18-30, must be non-smoker
• Willing to fast for 8 hours prior to a blood draw; Exclusion Criteria:- History of cosmetic surgery
• Use of anti-aging medications within 3 months of enrollment
• Use of over-the-counter anti-aging creams (not sunscreens) more than once per week within one month of enrollment
• Use of dietary supplements including vitamins within one month of enrollment
• Use of tanning beds or excessive UV exposure (greater than 2 hours per day) within one month of enrollment
• Use of sunless tanners within one month of enrollment
• Uncontrolled or ongoing serious medical condition
• Participation in another clinical study involving use of an investigational drug or product
• Medical condition which, at the discretion of the investigator, will have a significant impact on ability to judge participant's skin characteristics, including age.

Participants completing the protocol were included in the analysis.

Duration of enrollee participation (12 weeks)

All study participants were asked about adverse events at every study visit with results graded and recorded

Sample size limitation, short term nature of study